How to get Live Imaging of the Cellular Genome via CRISPR System?
The spatiotemporal organization of chromatin structure plays a critical role in regulating lineage-specific gene expression during cellular differentiation and embryonic development, via contributing to the functional output of the genome, which can be dynamically amplified or suppressed. However, there is little known about how the genomes being modified and how their structural organization regulating function output.
Answer:
a catalytically inactive Cas9 enzyme (dCas9) is fused to fluorescent labels (e.g., GFP)
Explanation:
The CRISPR-Cas9 genome editing system consists of two parts: 1- a Caspase enzyme called 'Cas9' with catalytic activity that cuts DNA strands by hydrolyzing phosphodiester bonds, and 2-a single guide RNA (sgRNA) that directs Cas9 to target sites with complementarity to nucleotides of the sgRNA in order to edit these sequences. Using a catalytically dead Cas9 (dCas9) it is possible to repurpose the CRISPR-Cas9 system, for example, by fusing dCas9 to epigenetic enzymes for epigenome editing or by fusing dCas9 to the Green Fluorescent Protein (GFP) for imaging of the cellular genome. Thus, it is possible to use dCas9 to create a customizable DNA labeler that can be complemented with a fluorescence microscope and thus track multiple genomic loci in living cells.
Which statement discusses the gland(s) and hormone(s) that are most involved in regulating a fight-or-flight response? The pancreas produces insulin. The adrenal glands produce epinephrine. The ovaries produce estrogens. The parathyroids produce parathyroid hormone.
Answer:
b
Explanation:
What result allowed Dintzis to conclude that proteins are synthesized from the N terminus to C terminus
Answer:
The more newly synthesized peptides had greater levels of radiolabeled H
Explanation:
Using immature red blood cells (reticulocytes), Dintzis showed that polypeptide chains of hemoglobin were created from N-terminal to the C terminus during translation. Dintzis labeled with radioactive amino acids that were being actively translated, subsequently digested the translated proteins with trypsin and finally determined the amount of tryptic protein fragments. From this experiment, Dintzis observed that the radioactive products were concentrated in the C-terminus (i.e., labeled amino acids appeared first in the C terminal tryptic peptides), thereby showing that the direction of protein synthesis is from the N terminus to the C terminus.
Need help please and thanks
Answer:
The concentration of the oxygen is higher outside of the cell and the oxygen will move inside or towards the cell since it is higher in the outside and lower inside the cell and the transport is passive and it doesn't require energy.
The vertical acis on a graph is called the y-axus for scientific data what usually placed along the y-axis
Answer:
dependent variables
What happens to animals because of plastic trash in the ocean ?
Answer:
They suffocate and choke because of the plastic waste, they also feel trapped in there and sometimes smaller animals get stuck in there
Answer:
Fish, seabirds, sea turtles, and marine mammals can become entangled in or ingest plastic debris, causing suffocation, starvation, and drowning. ... Plastic waste kills up to a million seabirds a year. As with sea turtles, when seabirds ingest plastic, it takes up room in their stomachs, sometimes causing starvation.
Explanation:
Which word best describes the response the seedlings are shown?
Which statement best describes the components of nucleic acids?
Answer:
The statement which describes best about nucleic acids is: They are made up of 'nitrogen bases', 'sugars' and 'phosphates'.
Explanation: Nucleic acids are biomolecules that are known to all forms of life.
g The lac operon - TRUE or FALSE Before the induction of the lac operon, lactose transporters are already present in the cell membrane. TRUE Lactose initially enters the cell using a transport protein.
Answer:
True
True
Explanation:
Lactose transporter are not absent from the membrane before induction. They are already present in the cell membrane. Lactose requires some transport medium, for this reason protein acts as transport medium for the lactose.
state the role of alcohol in this investigation
The dissolved waste molecules produced by the cells are removed primarily from the blood by the what?
A. large intestine
B.kidneys
C. skin
D. lungs
Which of the following cells do not have specialized tasks in an organism? 1.) prokaryotic cells 2.) eukaryotic cells 3.) animal cells 4.) plant cells
Answer:
A. Prokaryotic cells
Explanation:
Prokaryotic cells don't have organelles
what kind of graph is this?
Answer:
Explanation:
financial chart
In which direction will the object pictured below be accelerating?
In the picture below the purple circles represent salt molecules. Which way would
the water molecules move across the membrane
Right to left toward the higher concentration of salt
Left to right away from the higher concentration of Salt
pls help!!!! +10 brainly points
(I put a random answer in)
_________ is a form of spina bifida in which a saclike cyst filled with CSF protrudes through the spinal defect but does not involve the spinal cord. a. Meningomyelocele b. Meningocele c. Myelomeningocele d. Spina bifida occulta
Answer:
b. Meningocele
Explanation:
Meningocele is a congenital deformity where there is a sac protruding from the spinal column, which is not related to acute neurological conditions. Thus, meningocele is caused by a protrusion of the meninges (i.e., membranes that envelop the brain and spinal cord) and cerebrospinal fluid but without accompanying nervous tissue. About 1,500 babies each year born with this type of spinal birth defect. Moreover, spina bifida occulta is a mild type of spina bifida caused when one or more vertebrae do not fully form during pregnancy. Finally, meningomyelocele (also known as myelomeningocele) is a severe type of spina bifida caused when the spinal cord and nerves develop outside of the body.
Answer:
b. Meningocele
Explanation:
Meningocele is a form of spina bifida in which a saclike cyst filled with CSF protrudes through the spinal defect but does not involve the spinal cord.
The diagram below shows two locations where fossils of Mesosaurus have been found. Mesosaurus was a fresh water reptile that existed on Earth about 250 million years ago. Which statement best explains why these freshwater Mesosaurus fossils are found today in some rock layers in both South America and Africa?
Answer:
they was together at one point but because of the plants under the Continent they were separated and moved
The statement which best explains why these freshwater Mesosaurus fossils are found today in some rock layers in both South America and Africa is The remains of the Mesosaurus were carried across by predators.
Where have Mesosaurus fossils been found?
Remains of Mesosaurus, a freshwater crocodile-like reptile that lived during the early Permian (between 286 and 258 million years ago), are found solely in Southern Africa and Eastern South America. It would have been physiologically impossible for Mesosaurus to swim between the continents.
How do we know Mesosaurus was freshwater?For years, mesosaurs have been considered aquatic (water-living) animals, because they have several characteristics of species that are adapted to aquatic life, like webbed hands and feet and the presence of thickened and compact bones.
Learn more about Mesosaurus at https://brainly.com/question/9647452
#SPJ2
Particulates can be removed from smokeslack emissions by which of the following methods?
Help please! The question is in the image below
Answer: A. The cells produced sugar and oxygen during photosynthesis
Explanation: Plants today make use of this same trait, absorbing the sun's energy and air to create food (sugar) and oxygen (as a by-product)
In guinea pigs, the color of the fur is determined by a single gene with two alleles control. The brown allele (B) is dominant over the white allele (b). Two heterozygous guinea pigs are crossed. Draw a genetic diagram with the genotypic, phenotypic and ratio information of the P₁ and F ₁ generations of this cross.
Answer:
See
Explanation:
Given:
Brown allele (B) is dominant over the white allele (b).
It will be assumed that the alleles are not related to the X and Y chromosomes.
See attached diagram for details of the general case (for both homo-zygous and hetero-zygous parents)
For both parents hetero-zygous,
P1:
Genotype: Bb (100%)
Phenotype: brown (100%)
F1:
Genotypes : BB, Bb, bB and bb in equal proportions, i.e.
BB (25%), Bb (50%), bb (25%)
Phenotypes: 75% brown (from BB and Bb), 25% white (from bb)
The condensed form of dna used for cell decision
Answer:
Chromatin
Explanation:
I'm pretty sure this is the answer, let me know if it isn't.
याेग्यबाबुकाे याेग्यपुत्रका रुपमा बहादुर शाहलाई मुल्याङ्कन गर्नुहाेस?
Explanation:
Bahadur Shah was the youngest son of King Prithivi Narayan Shah. He ruled as a regent for next 9 years. During his regency the border of Nepal was extended to Kumau Gadwaal. He was a visionary and courageous ruler. He married the Princess of Palpa with the intention of strengthening the ties between Nepal and also recruited qualified people in his army to make his army strong. In jestha 1843BS, he conquered Gulmi, Argakhaanchi, Dhurkot and included them in Nepal. He also achieved victory over Parvat in the Asoj of the same year. After that Pyuthan, Dang, Rolpa and Jajarkot also came under Nepal's rule.
There are three criteria that scientists use to classify kingdoms. What are they?
Answer: cell type, ability to make food, and number of cells in their body
Explanation:
According to Darwin, ______ in species could result from many ______ over long periods of time.
A. population growth; births
B. big changes; small changes
C. mating; interactions
D. xtinction; deaths
According to Darwin, [tex]\sf\purple{big\:changes}[/tex] in species could result from many [tex]\sf\pink{small\:changes}[/tex] over long periods of time.
B. big changes; small changes ✅
[tex]\circ \: \: { \underline{ \boxed{ \sf{ \color{green}{Happy\:learning.}}}}}∘[/tex]
What is the answer to number 1
Answer:
TTT GCC AGT
Explanation:
Can the body use heat energy to do work? if not what does the body use.
No links please
Answer:
Explanation: The body cannot use thermal energy but your body can release the chemical potential energy in your food without combustion.
. The area of particle 1 is
2.5 mm². The area of particle 2
is 0.5 mm². The area of particle
1 is how many times as big as
the area of particle 2?
Answer:
The correct answer is - 5 times
Explanation:
Given:
area of P1 = 2.5
area of P2 = 0.5
Solution:
Let assume:
area of P1 is x times of the area of P2
then the equation would be:
=> 2.5 = x*0.5
=> 2.5/0.5 = x
=> 5 = x
Thus, area of P1 is 5 times to the area of P2
___ A mismatched base pair during DNA replication B. Photoreactive repair ___ A thymine dimer in a bacterial cell A C. NHEJ repair ___ A mismatched base pair that occurred after DNA replication D. Base excision repair ___ A double strand break in G1 (cell does not care about precise repair) E. HR repair ___ A thymine dimer in a eukaryotic cell F. SDSA repair ___ A double strand break in G1 (cell does care about precise repair)
Answer:
Explanation:
●photoreactive repair - A thymine dimer in a bacterial
●SDSA -A mismatched base pair during DNA replication
●. Photoreactive repair - A thymine dimer in a eukaryotic cell
● NHEJ repair- double strand break in G1 (cell does care about precise repair)
100 POINTS!! Please answer all questions, will report you if you don't give a serious answer
Answer:
1. b
2. l
3. i
4. c
5. k
6. y
Explanation: