Which column would you use to purify a 32kd positively charged tagged protein from a 35kd negatively charged protein?
a. G200 gel filtration column
b. G100 gel filtration column
c. Ni+2 Agarose
d. ion exchange column
The most appropriate column to purify a 32 kDa positively charged tagged protein from a 35 kDa negatively charged protein would be the ion exchange column. The correct option is d.
Ion exchange chromatography separates proteins based on their net charge. In this case, the positively charged tagged protein can bind to a negatively charged ion exchange resin, while the negatively charged protein will not interact significantly and can be washed away. The bound protein can then be eluted using a salt gradient or a pH change.
The G200 and G100 gel filtration columns (a and b) are used for size-based separation, where proteins are separated based on their molecular weight. The molecular weights of both the target protein and the contaminating protein are close, and the charge difference between them is the key distinguishing factor. Therefore, gel filtration columns would not effectively separate the two proteins.
Ni+2 Agarose (c) is typically used for purification of His-tagged proteins through affinity chromatography. Since the target protein in this case is not His-tagged and does not have an affinity for Ni+2, this column would not be suitable for separating the target protein from the contaminating protein.
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4 reasons why we should apply tertiary early
Answer:
you stand a better chance of being accepted at tertiary without any hassles.
you'll be able to have better piece of mind.
The brain recognizes action potentials from different sensory modalities as separate and distinct because Group of answer choices the volley of action potentials differs for each sensory modality. action potentials from different sensory modalities are processed through different frontal lobe regions. action potentials from different sensory modalities utilize different neurotransmitter systems. action potentials from different sensory modalities are carried on different nerve tracts.
Answer:
The correct answer is ''action potentials from different sensory modalities are carried on different nerve tracts.''
Explanation:
A nerve tract is a set of axons (bundles of nerve fibers) of the central nervous system that have the same origin and the same destination. Each of the main sensory types that we can experience, pain, touch, vision, sound, etc., is called sensation modality. Nerve fibers only transmit action potentials whatever the stimulus. The perceived modality will depend on the specific point in the central nervous system where the excited fiber ends, that is, the response indicates that each nerve bundle ends at a specific point in the central nervous system and the type of sensation experienced when a nerve fiber is stimulated is determined by the area of the nervous system to which this fiber leads. For example, if a fiber is stimulated for pain, the person perceives this sensation regardless of the type of stimulus that excites them. It can be electricity, overheating the fiber, crushing it, or activating the nerve ending for pain when tissue cells are injured. In all these cases, the person perceives pain. Similarly, if a tactile fiber is stimulated by electrical excitation of a touch receptor or by some other mechanism, the person perceives a sensation of touch because these fibers lead to specific areas of touch in the brain. In this same sense, the fibers from the ocular retina end in the visual areas of the brain, those from the ear end in the auditory areas and the thermal fibers in the areas for temperature. This specificity of nerve fibers to transmit nothing more than a sensation modality is called the principle of the marked line.
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What is H²O?
H2O is the chemical formula for water, meaning that each of its molecules contains one oxygen and two hydrogen atoms H.
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H²O is the chemical formula of water.
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Which of the following statements about insulin is true?
A. Insulin acts as a transport protein, carrying glucose across the cell membrane.
B. Insulin facilitates the movement of intracellular glucose transporters to the cell membrane.
C. Insulin stimulates the breakdown of stored glycogen into glucose.
D. Insulin stimulates the kidneys to reabsorb glucose into the bloodstream.
E. Two of the above are true statements.
The correct option is B. Insulin facilitates the movement of intracellular glucose transporters to the cell membrane.
The cell membrane, also known as the plasma membrane, is a vital component of all living cells. It is a thin, semi-permeable barrier that surrounds the cell, separating the internal cellular environment from the external surroundings. Composed primarily of lipids, proteins, and carbohydrates, the cell membrane acts as a protective layer, regulating the flow of substances in and out of the cell.
The lipid bilayer is the fundamental structure of the cell membrane, with hydrophilic heads facing outward and hydrophobic tails facing inward, creating a stable barrier. Embedded within this lipid bilayer are various proteins that serve numerous functions, including transport of molecules, cell signaling, and cell recognition.
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A geneticist crossed fruit flies to determine the phenotypic ratio. The geneticist crossed a fly with blistery wings and spineless bristles (bbss) with a heterozygous fly that had normal wings and normal bristles (BbSs). Which proportion of offspring that are dominant for both traits in would you NOT expect based on Mendel's law of independent assortment
Complete question:
A geneticist crossed fruit flies to determine the phenotypic ratio. The geneticist crossed a fly with blistery wings and spineless bristles (bbss) with a heterozygous fly that had normal wings and normal bristles (BbSs). Which proportion of offspring that are dominant for both traits in would you not expect based on Mendel's law of independent assortment? 1/2 , 4/16, 25% , or 1/4
Answer:
1/2 is the proportion of the offspring that is NOT expected among individuals that are dominant for both traits.
4/16 = 1/4 = 25% of the progeny and the correct expected proportion of individuals that are dominant for both traits.
Explanation:
Available data:
Cross: a fly with blistery wings and spineless bristles with a heterozygous fly that had normal wings and normal bristles Recessive trait: blistery wings and spineless bristlesDominant trait: normal wings and normal bristlesLet us say that:
B is the dominant allele for normal wingsb is the recessive allele for blistery wingsS is the dominant allele for normal bristless is the recessive allele for spineless bristlesParentals) bbss x BbSs
Gametes) bs, bs, bs, bs BS, Bs, bS, bs
Punnett square) BS Bs bS bs
bs BbSs Bbss bbSs bbss
bs BbSs Bbss bbSs bbss
bs BbSs Bbss bbSs bbss
bs BbSs Bbss bbSs bbss
F1) 4/16 = 1/4 = 25% of the progeny is expected to be BbSs, dyhibrid individuals, expressing normal wings and normal bristles
4/16 = 1/4 = 25% of the progeny is expected to be Bbss, expressing normal wings and spineless bristles
4/16 = 1/4 = 25% of the progeny is expected to be bbSs, expressing blistery wings and normal bristles
4/16 = 1/4 = 25% of the progeny is expected to be bbss, expressing blistery wings and spineless bristles
Which of the following applications of genetic engineering is preventative and helps
individuals fight infection before its onset?
A)Insulin production
B)Vaccine production
C)Stem cell therapy
D)Gene therapy
is the cell membrane organelle, cell tissue, organ, organ system organismis
Answer:
The major levels of organization in the body, from the simplest to the most complex are: atoms, molecules, organelles, cells, tissues, organs, organ systems, and the human organism.
Explanation:
Why is photosynthesis limited to surface water in marine ecosystems?
A. Visible light cannot penetrate deeply into the ocean, even when the water is clear.
B. The temperature is too cold in deep oceans for plants to grow.
C. Volcanic gases can seep through the ocean floor through cracks called "hydrothermal vents," which makes life impossible in the deep ocean.
D. Inorganic nutrients that plants need to grow are found mostly near the surface.
Photosynthesis is limited to surface water in marine ecosystems due to the visible light cannot penetrate deeply into the ocean, even when the water is clear. The correct answer is option A.
Photosynthesis is the process by which plants, algae, and some bacteria transform light energy into chemical energy. In this process, carbon dioxide and water are combined to create organic compounds such as glucose, with oxygen as a byproduct. Photosynthesis in marine ecosystems is primarily limited to surface waters due to the inability of visible light to penetrate deeply into the ocean, even when the water is clear.
Sunlight contains the necessary energy for photosynthesis, and the wavelengths of light required for this process are absorbed by water molecules as they travel through the ocean. As a result, the available light diminishes rapidly with increasing depth, making it insufficient for photosynthesis to occur effectively in the deeper parts of the ocean. This is why photosynthetic organisms, such as algae and phytoplankton, are primarily found in the sunlit upper layers of marine ecosystem.
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Which phyla have well developed nervous system
Explanation:
Cnidarians
.............
kinetic energy is a function of
A. the Kelvin temperature scale
B. direction
C. velocity
La influencia que tiene la temperatura en la reproducción de los paramecios, en muestras de agua estancada
Answer:
Para un paramecio la temperatura óptima requerida para llevar a cabo sus funciones metabólicas (incluida la reproducción) se encuentra entre 24 °C y 28 °C
Explanation:
Los paramecios (género Paramecium) son protozoarios, es decir, organismos microscópicos del reino protista. Un paramecio es un organismo unicelular que puede llevar a cabo todas las funciones esenciales de un modo funcionalmente similar a una célula animal. Los paramecios pueden reproducirse tanto de manera sexual como asexual (bipartición). Estos organismos viven en cuerpos de agua dulce rica en nutrientes orgánicos como estanques, charcos, regiones de ríos parcialmente estancadas, etc. En estos ambientes los paramecios se reproducen de un modo óptimo a una temperatura entre 24 °C y 28 °C, donde pueden llegar a dividirse hasta 4 veces al día.
How is ADP
converted to ATP?
Answer:
ADP is converted to ATP for the storing of energy by the addition of a high-energy phosphate group. The conversion takes place in the substance between the cell membrane and the nucleus, known as the cytoplasm, or in special energy-producing structures called mitochondria.
Explanation:
true/false. there are three children, three pets, and three favorite colors. the children are: angela, lisa, and susan. the pets are: a cat, a dog, and a fish. the colors are: blue, green, and red.
The statement is true. There are three children (Angela, Lisa, and Susan), three pets (a cat, a dog, and a fish), and three favorite colors (blue, green, and red) mentioned in the description.
The statement clearly states that there are three children, three pets, and three favorite colors. It specifies the names of the children as Angela, Lisa, and Susan, the pets as a cat, a dog, and a fish, and the favorite colors as blue, green, and red. The information provided matches the given quantities and names, confirming that there are indeed three children, three pets, and three favorite colors as mentioned in the statement. Therefore, the statement is true.
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The ecosystem with the greatest sustainability will be the one that has the
Answer:
Different organisms or animals
Explanation: In a ecosystem, if you have different animals or organisms, it give them a better chance of survival, reason being that they can choose to eat whatever animal or organism they want because there is lots of animals to choose from the ecosystem. Basically the more diversity you have, the better chance you will survive, and the less diversity you have, the less chance of survival.
in peas, both male and female sex cells -blank- are in the same flower
Which of the following accurately contrasts genotype and phenotype?
1. A genotype is traits that are passed on genetically, while a phenotype is traits that are developed environmentally.
2. A genotype is genetic traits that are inherited from the father, while a phenotype is traits passed from the mother.
3. A genotype is genetic traits that cannot be observed, while a phenotype refers to observable traits.
4. A genotype is traits that are not predictable, while a phenotype refers to traits that can be predicted.
Answer:
3.
Explanation:
genotypes are traits embedded in the genes that cannot be observed whilst, a phenotypes are traits that are physically displayed by the body and therefore, can be observed.
example,
genetic factor - brown colored eyes.
connotation for the gene - B
Genotype - BB.
phenotype - brown colored iris.
hope it helps. :)
A _______ is a substance containing incomplete portions of a virus that produce an immune response when injected into the bo
A vaccine is a substance containing incomplete portions of a virus that produce an immune response when injected into the body."
A vaccine is a type of biological preparation that improves immunity to a specific disease. It typically consists of an agent that resembles a disease-causing microorganism and is often made from weakened or killed forms of the microbe, its toxins, or one of its surface proteins.
There are different types of vaccines such as inactivated vaccines, live attenuated vaccines, subunit vaccines, conjugate vaccines, and DNA vaccines. They all work by stimulating the immune system to recognise and attack specific viruses or bacteria.
The "substance containing incomplete portions of a virus" mentioned in the question refers to the antigenic components of a vaccine. These antigens are usually derived from the surface proteins of a virus and are used to trigger an immune response when injected into the body. Once the immune system recognises the vaccine antigen as foreign, it generates an immune response that involves the production of antibodies and the activation of immune cells. This immune response results in the development of immunological memory that helps the body to quickly recognise and respond to the actual virus if it is encountered in the future.
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Because stress is a force, it... A) takes energy out of rock B) adds energy to rock C) adds volume to rock. D) makes rock harder.
Answer:
it adds energy to the rock
Explanation:
Because stress is a force, it adds energy to the rock. This stress/force is being applied to the rock. Whether the rock moves or not you are applying a force to the rock and it has potential energy. If the rock moves, you are adding energy to it and creating kinetic energy as the rock moves. Other forces such as gravity and friction can push against your force and prevent the rock from moving. Therefore, there are many forces at play in such a scenario.
how does the antigen-antibody complex protect the body
Answer:
Explanation:
the antibodies destroy the antigen (pathogen) which is then engulfed and digested by macrophages. White blood cells can also produce chemicals called antitoxins which destroy the toxins (poisons) some bacteria produce when they have invaded the body.
which ocean zone has the most abundant primary producers and why
Answer:
the epipelagic zone ,due to its high light availability and warm water temperatures.
The law of conservation of energy states that energy cannon be created or destroyed.
O True
O False
Answer:
false
Explanation:
The first law of thermodynamics, also known as Law of Conservation of Energy, states that energy can neither be created nor destroyed; energy can only be transferred or changed from one form to another. For example, turning on a light would seem to produce energy; however, it is electrical energy that is converted.
Hope this helps get an A for me kay!!!
Is DNA replication always a foolproof process? Explain your answer.
Answer: No. Although many proteins check the DNA for damage or errors, damaged regions can still be replicated. This may result in gene alterations and serious complications for the organism.
Explanation:
No, replication is not always a foolproof process although many proteins look for DNA damage or mistakes, damaged areas can still replicate. This can lead to gene rearrangements and serious problems in the body.
Some mutations are caused by mistakes in DNA replication. DNA replication isn’t always 100% complete. Sometimes, an incorrect base is inserted into the new DNA strand. This incorrect base can become permanent.
Breaks in DNA are caused by DNA replication errors, particularly those in regions that are difficult to replicate, known as fragile sites.
Breaks in DNA can cause cancer, mainly because they increase the risk of chromosome fragments rearranging themselves and activating genes that cause uncontrolled cell division.
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If vegetation has 10000 kcal , what will be the energy of tertiary level as per 10% energy rule
Answer: If a grassland ecosystem has 10,000 kilocalories (kcal) of energy concentrated in vegetation, only about 1,000 kcal will be transferred to primary consumers, and very little (only 10 kcal) will make it to the tertiary level.
Explanation: Hope it helped
Starfish are echinoderms, a term that means spiny skin. The rough covering serves as a natural barrier and protects the starfish from predators and the environment. What system includes skin?
what is the difference between a cell and a cell membrane
P.S. ENJOY THE FREE POINTS /100 POINTS
Answer:
The cell wall is mainly present in plant cells while the cell membrane is present in the animal cell. The cell wall is made of cellulose whereas cell membrane lipids and proteins The cell wall is completely permeable while the cell membrane is semi-permeable
The cell wall is mainly present in plant cells while the cell membrane is present in the animal cell. The cell wall is made of cellulose whereas cell membrane lipids and proteins The cell wall is completely permeable while the cell membrane is semi-permeable.
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arrange the following structures in the order in which blood flows through them, starting with the right atrium
The correct order in which blood flows through the structures, starting with the right atrium, is as follows: right atrium, tricuspid valve, right ventricle, pulmonary valve, pulmonary artery, lungs (pulmonary circulation), pulmonary veins, left atrium, mitral valve, left ventricle, aortic valve, aorta, and the systemic circulation.
The flow of blood starts in the right atrium, where deoxygenated blood returns from the body. From the right atrium, the blood flows through the tricuspid valve into the right ventricle. The right ventricle then pumps the blood through the pulmonary valve into the pulmonary artery, which carries the blood to the lungs for oxygenation. After oxygenation in the lungs, the blood returns to the heart through the pulmonary veins, entering the left atrium. From the left atrium, the blood flows through the mitral valve into the left ventricle. The left ventricle pumps the oxygenated blood through the aortic valve into the aorta, the largest artery in the body, initiating the systemic circulation that supplies oxygenated blood to all tissues and organs. Blood flow through the heart follows a specific pathway to ensure efficient oxygenation and circulation throughout the body. The right atrium receives deoxygenated blood from the body through the superior and inferior vena cava. From the right atrium, the blood passes through the tricuspid valve into the right ventricle. The right ventricle contracts, pumping the blood through the pulmonary valve into the pulmonary artery. The pulmonary artery carries the deoxygenated blood to the lungs, where it undergoes oxygenation and releases carbon dioxide.
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Give the Correct Title for the following labeled diagram
I’ll MARK BRAINLIEST TO WHOEVER ANSWES FIRST
Answer:
This is the rock cycle I believe hope this helps
. ___________________ is the process of plants releasing oxygen into the environment?
Answer:
photosynthesis
Explanation:
can i get brainlists
What Phenotypic Ratio would you expect to result from a cross between 2 plants that are both Heterozygous for height and seed color? Ex) HhYy X HhYy
A). 1:2:1
B).4:4:4:4
C).8:8
D).9:3:3:1