The enzyme provided is a stock solution which should be diluted 50-fold with 0.001M HCl immediately before use.

Enzyme :1mg/ml chymotrypsin A in 0.001M HCl



How do I work out this calculation, if I want the 5ml of the diluted enzyme?

Answers

Answer 1

In order to prepare a 50-fold diluted enzyme, 0.1 mL of the stock solution of the enzyme is taken and made up to 5.0 mL with 0.001M HCl.

What is dilution?

Dilution is a process whereby a solution of lower concentration is prepared from one of a higher concentration.

Dilution is done by using the dilution formula below:

C1V1 = C2V2

C1; Initial concentration of enzyme = 1 mg/mL

C2; Final concentration of enzyme = 1/50 mg/ml = 0.02 mg/ml

V1; Initial volume of enzyme = y

V2; Final volume = 5.0 ml

V1 = C2V2/C1

V1 =  0.02 * 5/1 = 0.1 mL

Therefore, in order to prepare a 50-fold diluted enzyme, 0.1 mL of the stock solution of the enzyme is taken and made up to 5.0 mL with 0.001M HCl.

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Related Questions


1. Write an unbalanced equation to represent each of the following equations. (Make sure to
show diatomic elements.)
(a) iron and sulfur react to form iron(II) sulfide
(b) hydrogen gas and oxygen gas react to form water
(c) aluminum oxide reacts to form aluminum and oxygen gas
(d) zinc and copper(II) sulfate react to form zinc(II) sulfate and copper
(e) potassium hydroxide and hydrogen bromide react to form potassium bromide and
water

Answers

The equation for each reaction is written according to the relevant as below.

Equations of chemical reactions

The reaction between iron and sulfur to form iron (II) sulfide is written as:

  [tex]Fe + S --- > FeS[/tex]

The reaction between hydrogen gas and oxygen gas to form water is written as:

[tex]H_2 (g) + O_2 (g) --- > H_2O (l)[/tex]

The reaction of aluminum oxide to form aluminum and oxygen gas is written as:

[tex]Al_2O_3 --- > Al + O_2[/tex]

Zinc and copper(II) sulfate react to form zinc(II) sulfate and copper as follows:

[tex]Zn+CuSO_4--- > ZnSO_4+Cu[/tex]

Potassium hydroxide and hydrogen bromide react to form potassium bromide and water as follows:

[tex]KOH + HBr --- > H_2O + KBr[/tex]

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100.0 degrees C a sample of 750mL gas is found. If the temperature of the gas is increased by 300 degrees, what will be the new volume gas

Answers

100.0 degrees Celsius = 100.0 + 273 = 373.0 K

[tex]\frac{750}{373.0}=\frac{V_{2}}{673.0} \\ \\ V_{2}=\frac{(673.0)(750)}{373.0} \approx \boxed{1350 \text{ mL}}[/tex]

A certain atom has 22 protons and 19 electrons. This atom loses an electron. The net charge on the atom is now 4+. If this same atom with 22 protons and 19 electrons were to gain 3 electrons, the net charge on the atom would be

Answers

Answer: 0

Explanation:

If 3 electrons were to be gained, then the number of protons would equal the number of electrons.

What most likely happens when water vapor cools?

It changes into gas.
It changes into liquid.
Its temperature increases.
Its temperature remains constant.

Answers

Answer:

hello :)

Explanation:

Water vapor in the air reaches its dew point as it cools in the air around the can, forming liquid drops of water. Condensation is the process where water vapor becomes liquid.

Which of the following shows the wavelengths of light that an atom gives off when an electron falls to a lower energy level?
O A. Absorption spectrum
O B. Quantum spectrum
O C. Electromagnetic spectrum
O D. Emission spectrum​

Answers

The wavelengths of light that an atom gives off when an electron falls to a lower energy level corresponds to Emission spectrum , Option D is the correct answer.

What is Emission Spectrum ?

Light is absorbed or emitted when an electron jumps or falls into an energy level.

The energy of light absorbed or emitted is equal to the difference between the energy of the orbits.

Therefore , the wavelengths of light that an atom gives off when an electron falls to a lower energy level corresponds to Emission spectrum.

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Calculate the mass of 162 L of chlorine gas, measured at STP.

Answers

Answer:

V=162 l

Vm=22.4 mol/l

n=V/Vm=7.23 moles

n=m/M M Cl=35.4

m=n×M

m=256.01 grams

Which of the following is true about homogeneous mixtures?

a) They consist of two or more phases.
b) They are always liquids.
c) They have compositions that never vary.
d) They are known as solutions.

Answers

the answer is that d is true and the correct answer

An acid is used to bring the pH of a solution from pH 9 to pH 6. How many times more acidic is the final solution than the initial solution?

Select one:

a.
3


b.
10


c.
100


d.
1000

Answers

Bleh bleh bleh bleh bleh bleh bleh bleh and bleh

Pulses A, B, C, and D all travel at 10 m/s on the same string but in opposite directions. The string is depicted at time t=0 in (Figure 1). The small pulses have an amplitude of 2.0 cm, and the large pulse has an amplitude of 4.0 cm.

A) Find the displacement of point P at time t=0.10s .

B) Find the displacement of point P at time t=0.20s .

Answers

(a) The displacement of point P at time t=0.10s is determined as +2cm.

(b) The displacement of point P at time t=0.20s is determined as -2cm.

What is displacement?

Displacement is the change in position of an object. It is obtained from the product of velocity and time of motion.

x = vt

Displacement of the waves after 0.1 s

x = 10 m/s x 0.1 s = 1 m

Each wave will travel 1 m to the right or to the left, depending on the initial direction.

wave B from left will stop at point 0 mwave A from left will stop at point -1 mwave C from right will stop at point 0 mwave D from right will stop at point + 1 m

wave B and C superimposed and the displacement will be between A and D.

Amplitude of A = - 2cm

Amplitude of D = + 4cm

Displacement of point P = 4 cm - 2 cm =  2cm

Displacement of the waves after 0.2 s

x = 10 m/s x 0.1 s = 2 m

Each wave will travel 2 m to the right or to the left, depending on the initial direction.

wave B from left will stop at point 1 mwave A from left will stop at point 0 mwave C from right will stop at point -1 mwave D from right will stop at point 0 m

Displacement of point P = (amplitude B + amplitude C) + (amplitude A + amplitude D)

Displacement of point P= (2cm - 2cm) + (2 cm - 4cm)= -2cm

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considering the temperature vs. time graph below, how does the temperature at the beginning of a change of state
ompare with the temperature at the end of the change?

O The temperature is always lower.
O The temperature is always the same.

O The temperature is usually lower.
The temperature is usually higher.

Answers

Answer:

temp is same

Explanation:

The change of state portionsof the graph are at 2  and 4....and the temp is the same at the beginning and ends.

 change of state is a constant temp process

What is the volume of a 12.96 g sample of pure hydrogen gas?

Answers

The volume of the 12.96 g sample of pure hydrogen gas is 145.24 L

How to determine the mole of hydrogen gas

We'll begin by calculating the number of mole in 12.96 g hydrogen gas. This can be obtained as follow:

Mass of Hydrogen gas = 12.96 gMolar mass of hydrogen gas = 2 g/mol Mole of Hydrogen gas =?

Mole = mass / molar mass

Mole of Hydrogen gas = 12.96 / 2

Mole of Hydrogen gas = 6.48 mole

How to determine volume of hydrogen gas

To determine the volume of the pure hydrogen gas, we shall assume that the gas is at standard temperature and pressure (STP). Thus, we shall apply the ideal gas equation as illustrated below:

Temperature (T) = STP = 273 KPressure (P) = STP = 1 atm Gas constant (R) = 0.0821 atm.L/Kmol Number of mole (n) = 6.48 moleVolume (V) =?

V = nRT / P

V = (6.48 × 0.0821 × 273) / 1

V = 145.24 L

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Part E
Tsunami evacuation plans often consider the elevation of the community and its distance from the shoreline when
deciding which towns to evacuate. How could this data help in building new homes and communities along coastal areas
that are prone to tsunamis?

Answers

Answer:

make the house elevated

Select the correct answer.
Which of the following is a physical model of the Sun?
O A.
an equation that describes the Sun's motion
OB.
a chart that lists the temperatures of different parts of the Sun
O C.
a computer program that shows how the Sun changes over time
O D. a paragraph that describes the Sun's structure
OE.
a small yellow ball that represents the Sun

Answers

E. a small yellow ball that represents the Sun

hope this helps!!

In an experiment, the molar mass of the compound was determined to be 118.084 g/mol. What is the molecular formula of the compound?

Answers

Answer:

Succinic acid

Explanation:

The most common possibility is succinic acid

As it has decimals after whole no till hundredth it contains OH and C in most of the cases .

Let's check for succinic acid

C4H_6O_64(12)+4(16)+664+48+6118u

Yes approximately equal

Molecular formula is.

(CH_2)_2(CO_2H)_2

Balance this reaction.


CO +
H2 →
CH3OH

Answers

1 Co + 2 H2 > CH3OH

That’s the balance equation

What is the molality of 35 g acetone dissolved in 0.95 kg water? The molar mass of acetone is 58.1 g/mol.

Answers

The molality of 35 g acetone dissolved in 0.95 kg water is 0. 63 mol/kg

How to determine the molality

Using the formula:

Molality = number of moles of solute÷ mass of solvent

Note that number of moles = mass divided by the molar mass

mass of acetone = 35g

Molar mass of acetone = 58. 1g/mol

Number of moles of acetone = 35÷ 58.1 = 0.602 mole

Molality of acetone = 0. 602÷ 0. 95 = 0. 63 mol/kg

Hence, the molality of acetone is 0. 63 mol/kg

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how many moles are in 150 grams lf NaOH

Answers

Answer:

3.75 mole

Explanation:

molecular mass of NaOH = 39.97 gm

i.e,

==>39.97 gm NaOH contains 1 mole of NaOH

==>1 gm of NaOH contains 1/39.97 mole of NaOH

==>150 gm of NaOH contains (1/39.97) *150 mole of NaOH

==>150 gm of NaOH contains 3.75 mole of NaOH.

Two contribution of chemistry in clothing​

Answers

♨ANSWER♥

Textiles for clothes are also sometimes finished with chemicals, for example, to stiffen them and make them wrinkle-free. Chemicals in their nanoforms are helping make textiles more resistant to water, stains, wrinkles, bacteria and mould. Some of the chemicals can be harmful.

...hope this helps...

_♡_mashi_♡_

20.0 g of bromic acid, HBrO3, is reacted with excess HBr.

HBrO3 (aq) + 5 HBr (aq) → 3 H2O (l) + 3 Br2 (aq)

What is the theoretical yield of Br2 for this reaction? Round your answer to the nearest gram.

Answers

After Rounding off The percentage yield is 64%

What is Percentage Yield ?

It is the ratio of actual yield to theoretical yield multiplied by 100% .

It is given in the question

20.0 g of bromic acid, HBrO3, is reacted with excess HBr.

The reaction is

HBrO₃ (aq) + 5 HBr (aq) → 3 H₂O (l) + 3 Br₂ (aq)

Actual yield = 47.3 grams

Molecular weight of Bromic Acid is 128.91 gram

Moles of Bromic Acid = 20/128.91 = 0.155 mole

Mole fraction ratio of Bromic Acid to Bromine is 1 :3

Therefore for 0.155 mole of Bromic Acid 3 * 0.155 = 0.465 mole of Bromine is produced.

1 mole of Bromine = 159.8 grams of Bromine

0.465 of Bromine = 74.31 grams of Bromine

Percentage Yield = (47.3/74.31)*100 = 63.65 %

After Rounding off The percentage yield is 64% .

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Answer:

74g

Explanation:

The other person had said that the Percentage Yield = (47.3/74.31)*100 = 63.65 %

So, since to find the percent yield you have to divide the actual yield by the theoretical yield, I would assume the theoretical yield is 74.31g (74g if you round to the nearest gram)

A helium balloon possesses a volume of 2500 L at a temperature of 114 °C. What volume will the balloon have if the temperature changes to 56 °C? The pressure remains constant

Answers

Given that the pressure remains constant, if the temperature of the helium balloon decreases to the given value, the volume increases to 2125.5L.

What is Charles's law?

Charles's law states that "the volume occupied by a definite quantity of gas is directly proportional to its absolute temperature.

It is expressed as;

V₁/T₁ = V₂/T₂

Given the data in the question;

Initial temperature of gas T₁ = 114°C = (114+273.15)K = 387.15KInitial volume of gas V₁ = 2500LFinal temperature T₂ = 56°C = (56+273.15)K = 329.15KFinal volume V₂ = ?

V₁/T₁ = V₂/T₂

V₂ = V₁T₂ / T₁

V₂ = ( 2500L × 329.15K ) / 387.15K

V₂ = 882875LK / 387.15K

V₂ = 2125.5L

Given that the pressure remains constant, if the temperature of the helium balloon decreases to the given value, the volume increases to 2125.5L.

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2) A solution of copper(II) chloride is added to a solution of sodium sulfide
A. Balanced equation:
B. Explanation using activity series and solubility rules as necessary:

Answers

The equation is CuCl2 + Na2S ------> 2NaCl + CuS because  Na is ahead of Cu in the electrochemical series.

What is a balanced reaction equation?

A balanced reaction equation is one in which the number of atoms of each element on the reactant side is equal to the number of atoms of the same element on the product side.

In this case, the balanced reaction equation is;

CuCl2 + Na2S ------> 2NaCl + CuS. The reaction occur thus because Na is ahead of Cu in the electrochemical series and must always displace it from its aqueous solution.

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A neutral atom of Sodium (Na) has an atomic number of 11 and an atomic mass of 23. Therefore, Na has _________________________ protons.

Answers

Answer:

11

Explanation:

Atomic number is the number of PROTONS an element has .

How do the valence electrons of an atom affect chemical reactions?

Answers

Answer:

Electrons of an atom can affect chemical reactions, because electrons have a negative charge, which can introduce a form of energy.

Why do you think there are many wind systems between a pole and the equator instead of just one large single wind system

Answers

As a result, the Poles have high pressure (cold air) while the Equator has low pressure (warm air). Hot air from the Equator rises and goes toward the Poles, while colder air from the Poles lowers and moves toward the Equator, resulting in a continuous wind pattern.

The kind of
reproduction that requires two
parents is called

What’s the answer for 3 and 8 please answer as soon as Possible due tomorrow morning.

Answers

Answer:

fgrthrdfghgfgfhfdhfdh

Explanation:

fdhhfgdghhdfghdfhgfhgfgh

answer number ! and fill in the unkown

Answers

The identity of the unknown compound is Urea from the Table# given. The reason for this identification will further be discussed below.

What is melting point?

Melting point can be defined as the point at which a substance changes from a solid phase or state to a liquid state

When two substances of different melting points are added together, the total melting point obtained is usually depressed.

From the table,

When urea and benzoic acid is added the melting point range becomes = 117-120

When urea and Cinnamic acid is added the melting point range becomes= 100-125, and both of which is lower than the individual melting points.

Therefore, the identity of the unknown compound is Urea from the Table# given.

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A crate holds 20 boxes of sewing supplies. Each box contains 8 spools of thread. Each spool has 250 meters of thread wrapped around it. What length of thread is contained in a crate?

Answers

Answer: 40000

Explanation:

250*8=2,000

2000*20=40000

chemistry can someone solve this?

Answers

eimetchuinsumuleavtubronAnswer:

Explanation:

All light rays parallel to the optical axis of a concave mirror are:
refracted.
reflected through the focal point.
diffused.
incandescent.

Answers

Answer:

A. Refracted

Explanation:

Incident rays parallel to the optical axis are reflected from the mirror.

PLEASE HELP!!!
Choose from the given list of aqueous solutions (A-D).

Solution A has [H3O+] = 3.5×10-5 M.
Solution B has [H3O+] = 3.5×10-4 M
Solution C has [OH-] = 1.3×10-10 M
Solution D has [OH-] = 6.2×10-11 M

Which solution is the least acidic?
Which solution has the highest pH?
Which solution is the most basic?
Which solution is the least basic?

Answers

Find pH of all

#A

pH=-log[H+]

pH=-log[3.5×10^{-5}]pH=-log3.5+5pH=4.4

#B

pH=-log3.5+4pH=3.4

#C

pOH=-log[OH]

pOH=-log[1.3×10^{-10}]pOH=-log1.3+109.8

pH=

14-9.84.2

#4

pOH=-log6.2+11pOH=10.2

pH

14-10.23.8

So our answers

solution A is least acidicsolution A has the highest pHsolution A is most basicsolution B is least basic

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