Quit Separation PAGE Fractions Help pH -01 6 7 00- 9 1 I 80K - 60K - 50K - 40K - 30K - M 20K - 15K- • 10K - 5K- 2D - PAGE of initial mixture Quit Separation PAGE Fractions Help pH 5 6 ~ N- 00- 9 1 1 1 80K - 60K - 50K 40K 30K - M 20KW 15K 10K 5K Using antibody to protein 4 Quit Separation PAGE Fractions Help Purification of protein 4 from Default Mixture Method Protein (mg) Enzyme (Units) Yield (%) Enrich Initial 511.0 7000.0 100.0 1.0 Records of subsequent purification steps will be added here. Cost (h/1000) 0 Protein 4 The enzymic activity of protein 4 is stable for several hours at temperatures up to 40°C and at pH values between 4.5 and 10. OK

Answers

Answer 1

This appears to be a record of a separation and purification process for a protein called "protein 4" using polyacrylamide gel electrophoresis (PAGE) and an antibody.

What is polyacrylamide gel electrophoresis?

Polyacrylamide gel electrophoresis (PAGE) is a method for separating and purifying different molecules, such as proteins and nucleic acids, based on their size and charge. It involves placing a sample in a well on a gel made of polyacrylamide, which is a type of polymer. An electric current is then applied to the gel, which causes the molecules to move towards the opposite charge.

Smaller molecules move more quickly through the gel than larger molecules, so they end up further down the gel. After the separation is complete, the different molecules can be visualized and collected. PAGE is often used in research and diagnostic laboratories to purify and analyze proteins and nucleic acids.

Learn more about PAGE, here:

https://brainly.com/question/5459997

#SPJ4


Related Questions

PLEASE HELP ASAP
Which of the following would provide the best evidence to support the claim that acid rain has a long-term effect on the stability of ecosystems?

Extinction of aquatic and terrestrial organisms would occur, decreasing species diversity and ecosystem stability.
The mutation rate would increase and new species would evolve, destabilizing ecosystems.
The pH level would increase and become intolerable to organisms, leading to decreased species diversity and ecosystem stability.
Habitat loss would occur, forcing organisms to adapt to new surroundings until the ecosystem eventually stabilized.

Answers

Answer:

A

Explanation:

Extinction of aquatic and terrestrial organisms would occur, decreasing species diversity and ecosystem stability.

name the part of the alimentary which absorbs a lot of water to produce solid waste from the food we eat

Answers

Answer:

Large intestine absorbs much water from food.It so essential for us to excrete waste products out of our body in the form of faecal matter.And main thing is water which is essential for us should not be excreted.So we get a dry faecal matter.Other wise if it fails to do a watery motion occurs

Explanation:

hope it helps

Are courgettes annuals or perennials? And why?

Answers

Answer:

Crops such as zucchinis and cucumbers are known as annuals because their natural life cycle only lasts a season.

Explanation:

A man with heart disease lives in a family that eats a lot of red meat. Wha

to his environment?

O Analyze the patient's family history regarding heart disease

O Analyze the patient's diet to quantify cholesterol intake

O Determine the exact amount of cholesterol in the patient's blood

O Determine if the cholesterol will lead to high blood pressure

Answers

Answer:

Analyze the patient's diet to quantify cholesterol intake

Explanation:

This is because since the patient family consumed more of red meat, it should be analysed. Because researches find out that red meat either processed or unprocessed contain more protein than fish, milk, and it can raise cholesterol levels. Red meat contain high cholesterol and increaee in cholesterol levels can lead to coronary heart diseases.

Answer:

B

Explanation:

Ernest was looking at the following diagram of a cell. Which statement best explains how he should classify the cell?


A. He should classify the cell as a plant cell because it has a cell membrane.

B. He should classify the cell as an animal cell because it has a flagellum.

C. He should classify the cell as a plant cell because it has cytoplasm.

D. He should not classify the cell as either a plant cell or an animal cell.

Answers

Answer:

C

Explanation:

I believe it is C because it looks like it has chloroplasts in it

"Evolution is like a climb up a ladder of progress; organisms are always getting better." O True O False​

Answers

Answer: True because In biology, evolution is the change in the characteristics of a species over several generations and relies on the process of natural selection. The theory of evolution is based on the idea that all species? are related and gradually change over time.

Even though the Earth's Inner core is hotter than the
liquid outer core, it is still solld because -

Answers

Answer: The inner core is solid because it is made of very dense, or heavy, materials - like iron and nickel. Even though it is very hot, these materials don't "melt" very easily, so they stay solid. Answer 3: It turns out that many materials can be a solid at a higher temperature if the pressure is also higher.

Explanation:

What are the 3 components/materials and energy needed to assemble carbohydrates?

Answers

Answer:

carbon, hydrogen, and oxygen.

Explanation:

Snapdragons show incomplete dominance for the flower color trait. A cross between snapdragon plants with red flowers and plants with white flowers produces F1 offspring with pink flowers.

When an F1 plant is self-pollinated, what percentage of its F2 offspring will be expected to have red flowers

Answers

Based on inheritance laws, it is expected none of the offspring will have red flowers.

What is incomplete dominance?

This occurs when a specific trait or allele is not completely expressed. In this case, the colors red and white are not completely expressed but they mix in the offspring.

What would be the dominant trait of the F2 generation?

Considering the pink flower will self-pollinate, it is expected the F2 generation has exactly the same traits as the F1 generation because there are no new genes or traits being combined. This means none of the F2 offspring will have red flowers, instead all plants will have pink flowers.

Learn more about dominance in: https://brainly.com/question/14053639

What does the stamen consist of:

(Check all that apply)

A. pistil


B. filament


C. ovary


D. anther

Answers

Answer:

Filament and Anther

Explanation:

What else should a grower do to make sure that the plants are photosynthesising at the optimum rate ?

Answers

Answer:

7 thats answer

Explanation:

Identify the preferable tonicity of these replacement drinks (isotonic, hypertonic or hypotonic)

Answers

Answer:

isotonic [same concentration]

hypotonic [low concentration]

hypertonic[high concentration ]

Am I healthy? Do you think so​

Answers

Maybe, haha. i dunno. why do you need to know?

☁️ Answer ☁️

U ARE SHININGGGGGG

But you shouldn't be posting ur pic on Brainly

In this phase, two nuclear membranes are reforming and the cytoplasm starts dividing.

Answers

Answer: Cytokinesis

Explanation:

the cytoplasmic division of a cell at the end of mitosis or meiosis, bringing about the separation into two daughter cells.

What is the relationship between a bee and flower

Answers

Answer:

Both are living organisms.

Both reproduce.

Both undergo growth and development.

Both react to changes to the environment.

Both excrete.

Explanation:

Someone please help ! No links please

Answers

No, the distance will change.

they don’t evolve around the sun in a circle. They evolved around the sun in an oval.

What is the relationship

Answers

Answer:

A

Explanation:

A

Which statement correctly describes a difference between asexual and sexual reproduction?

Answers

Answer:

B) Asexual reproduction involves one parent, and sexual reproduction involves two parents.

Explanation:

In asexual reproduction, offspring formed from only one parent whereas, in sexual reproduction, offspring produces from two parents. The offspring is identical to the parent in asexual reproduction while on the other hand, offspring is different from their parents. Sexual reproduction increases the chances of survival of the species due to genetic diversity whereas asexual reproduction has limited chances of survival due to lack of genetic diversity.

Asexual reproduction involves the production of offspring without the involvement of gametes, resulting in genetically identical offspring. On the other hand, sexual reproduction involves the fusion of gametes from two parents, resulting in offspring with genetic variation.

Asexual reproduction and sexual reproduction are two different methods of producing offspring in living organisms. Here are the differences between them:

1. Asexual reproduction:
Asexual reproduction involves the production of offspring from a single parent without the involvement of gametes (sex cells). This means that the offspring are genetically identical or clones of the parent.

Examples of asexual reproduction include binary fission in bacteria, budding in yeast, and regeneration in starfish. In binary fission, a single-celled organism divides into two identical cells. In budding, a small bud grows on the parent organism and eventually detaches to become a new individual. In regeneration, an organism can regrow a lost body part and develop into a complete organism.

2. Sexual reproduction:
Sexual reproduction involves the fusion of gametes from two parents to produce offspring. Gametes are specialized sex cells, such as sperm and eggs, which contain half of the genetic material of the parent.

In sexual reproduction, the offspring inherit genetic information from both parents, leading to genetic variation. This genetic diversity is important for the survival and adaptation of species over time.

Examples of sexual reproduction include humans, animals, and many plants. In humans, sperm from the male parent fertilizes an egg from the female parent, resulting in the formation of a zygote. The zygote then undergoes development to form a new individual with a unique combination of genetic traits.

To know more about Asexual reproduction:

https://brainly.com/question/4100787

#SPJ6

A student keeps a record of the phases of the moon for one month. On the first night of the student's observations, the full moon is visible in the sky. After the full moon, which of these phases will the student observe next?
F Waxing crescent moon
G Waxing gibbous moon
H Waning crescent moon
J Waning gibbous moon​

Answers

The answer is j :)))

Windy weather during summer season is
pleasant, why
?​

Answers

Answer:

because it cools you off when its too warm and overall gives a sense of calm

**PLEASE ANSWER!!! ILL CHOOSE BRAINLIESTT!**

Which of the following describes a mesopelagic zone?

a.) This ecosystem is in a transitional area that is influenced by the cycle between low and high tides.

b.) This zone measures from 600 to 3,000 feet below the surface, and is sometimes referred to as the “twilight zone” because of the amount of ambient light available.

c.) At the depths of 13,000 to 20,000 feet, this zone resembles a bottomless pit of complete darkness.

Answers

Answer: c

Explanation:

Identify the true statement about antiseptics, disinfectants, and antibiotics. Select one: a. Antibiotics are frequently targeted at a specific microorganism, whereas antiseptics and disinfectants are used to eliminate multiple organisms at a time. b. Antiseptics and disinfectants are not intended for internal use, but antibiotics are expected to reach the bloodstream to be effective. c. Antiseptics, disinfectants, and antibiotics eliminate microorganisms. d. All of these are correct.

Answers

Answer:

D all of these

Explanation:

what is electromagnetism​

Answers

Answer:

Electromagnetism is a branch of physics involving the study of the electromagnetic force, a type of physical interaction that occurs between electrically charged particles. ... At high energy the weak force and electromagnetic force are unified as a single electroweak force.

Can someone please help

Answers

2. Na=c, C=b, O=6 (just count the little dots bc those represent valence electrons)

3. A is false (noble gases for example don’t like to react after they have 8 valence electrons), B is true, and I think C is also true (it’s worded a little weird).

Which of the following is considered an ocean contaminant?
A)
sewage
B)
industrial waste
C)
fertilizer
D)
soaps
E)
all of the above

Answers

All of the above because a,b,c,d is not the answer

name some component of the blood that transports lactic acid

Answers

Answer:

plasma

Explanation:

the plasma circulates dissolved nutrients such as glucose, amino acids and fatty acids.

What motion does the graph above show the object during the time interval from 3
seconds to almost 4 seconds?

Answers

Answer:

I am not 100% sure but my best guess would be the object is moving backwards/falling.

Explanation:

The graph shows the position of the object going from almost 9.0 to below zero. There are many situations that could fit this. Such as, something going above and below sea level. OR something moving in front and behind a certain point. My best guess would be moving backward towards an object OR falling from a certain point.

why are viruses not placed into one of the five kingdoms​

Answers

Answer:

Viruses are microscopic organisms that are known to be the connecting link between living and non-living. These were not placed under the five-kingdom classification since they are neither living nor dead. Hence, they form their own group. Viruses are devoid of cells and cell organelles

Explanation:

describe various stages of mitosis

Answers

☁️ Answer ☁️

The Phases Of Mitosis

Interphase

Prophase

Metaphase

Anaphase

Telophase

cytokinesis

"Mitosis, which takes place in the cell nucleus, consists of many steps; these steps in turn, have several parts to them. The main steps in mitosis are: interphase, prophase, metaphase, anaphase, telophase and cytokinesis"

Hope it helps.

Have a nice day noona/hyung.

The difference between Secondary and Primary Succession is...
A. Primary only happens after a fire.

B. Secondary is starting from scratch with big rocks.

C. Secondary begins with soil, but no biotic (living) things.

Answers

Answer:

C.

Explanation:

This is because primary succession is newly formed logs that are colonized by living things. Seconday succession is there were living things there once but due to disturbance life is lost and started over which could take decades.

Answer:

C. Secondary begins with soil, but no biotic (living) things.

Explanation:

The difference between Secondary and Primary Succession is Secondary begins with soil, but no biotic (living) things.

Other Questions
Match the civilizations with their appropriate characteristics. 7(x+5) plese No links 1. What are the necessary criteria for proper measurement of an epicenter location? Arethere any limitations in this virtual lab that make finding the exact epicenter of anearthquake more difficult?2. How will knowledge of an earthquake epicenter help emergency management teamsmake better earthquake response plans? Find the median and Interquartile Range: 33, 42, 31, 27, 47, 23, 40, 45, and 43 A manager of a nursing home wants to be sure not to employ any immigrants who aren't authorized to work in the United States. The manager proposes that the company consider only job applicants who are obviously citizens. What is the legal problem with this idea? A. It is a violation of the WARN Act, which protects against discrimination B. It violates the principle of employment at will. C. It eliminates the need for requiring the Form I-9 verification process. D. It may lead to discrimination on the basis of race and national origin can someoneone help me with please i have to sent it todayWriting A storyRead the task below.Your English teacher has asked you to write a story for your school magazineThe story must begin with this sentence:It all began when Hayley was woken up by a loud noise.Your story must include: an open window a broken vaseWrite your story. If the same force is applied to an object with a large mass it will have a blank acceleration PLEASE HELP How many chlorine atoms are needed to satisfy the octet rule when forming an ionic compound with a cation of charge 3+? x-3=4x= ______explain Essay on Black Power Movement? 6. The United States was drawn into World War I mainly because of(1) exaggerated stories by yellow journalists(2) the unrestricted use of submarine warfare(3) a direct attack on an American military base(4) commitments made to the United Nations What new scientific beliefs emerged after WWI? Use a net of the square puritans if Jessica ty to find the surface area of pyramid 8 and 8 Select all the correct answers. Which two of these statements explains how can international trade agreements lead to economic growth? -By creating jobs in the export industries -By opening up new markets for domestic goods.-By reducing competition between domestic industries.-By reducing expenditures on domestic production. Please help Ill mark you as brainliest if correct The midpoint AB is M (1,1). If the coordinates of A are (-3,-3), what are the coordinates of B A key difference between the Roman Catholic Church and the churches of the Protestant Reformation was that, *A) unlike Protestants, Catholics usually believed in a non-hierarchical Church.B) unlike Catholics, Protestants usually believed that the Bible should be read in the local vernacular instead of Latin.C) unlike Catholics, Protestants usually believed that the Bible was the direct word of God.D) unlike Catholics, Protestants usually denied that God had three forms (Father, Son and the Holy Spirit). The Moon only emits light at night.TrueFalse - does not emit light at all Complete a departmental expense allocation spreadsheet for Marathon Running Shop. The spreadsheet should assign (1) direct expenses to each of the four departments, (2) the $64,000 of utilities expense to the four departments on the basis of floor space occupied, (3) the advertising departments expenses to the two operating departments on the basis of the number of ads placed that promoted a departments products, and (4) the administrative departments expenses to the two operating departments based on the amount of sales. what are the disadvantages of harbor sites.Grade 8 geo