Answer:
To give a correct answer, you'd have to describe yourself. But if you're asking me this question, then I'd say:
I am a outgoing, extroverted funny person. I am also considered artistic and smart. I often push boundaries of others and don't realize, yet I'm working on that.
Your six friends are now exploring Ellsmere Island in Canada. On this Island, in 2005, scientists
discovered the fossil of Tiktaalik roseag, a famous "transition fossil" between swimming fish and
four legged vertebrates (the tetrapods). Your friends begin to discuss Tiktaalik's (pronounced tik-
TAA-lik) origin.
This is what they said:
Answer:
transition fossil between swimming fish and four legged vertebrates
HELP IM ON LIMITED TIME!
Two closely-related insect species are being discovered.
are focusing on how long it takes each to develop limb buds
following fertilization. What evidence of evolution is this an example
of?
Select one:
Anatomical
Embryological
Molecular
DNA
Answer:
Embryological evidence
Explanation:
Embryological evidence is the type of evidence being discussed in the given scenario.
Embryological evidence of evolution refers to the similarities and differences observed in the early stages of development between closely-related species. In the given scenario, two closely-related insect species are being studied in terms of how long it takes each to develop limb buds following fertilization. This is an example of embryological evidence of evolution because it is concerned with the early stages of development in these species and how they may have evolved from a common ancestor.
Other types of evidence of evolution include anatomical evidence, which refers to the similarities and differences in the physical characteristics of closely-related species, and molecular evidence, which refers to the similarities and differences in the DNA and other molecules that make up an organism's genetic material.
Answer:
This is an example of embryological evidence for evolution
Explanation:
This is an example of embryological evidence for evolution. Embryological evidence for evolution refers to the study of the development of different species in order to understand their evolutionary relationships. In this case, the scientist is focusing on the development of limb buds in two closely-related insect species and comparing the length of time it takes for these structures to form. This information can be used to understand the evolutionary relationships between these species and provide evidence for the theory of evolution.
Within a cell, the amount of protein made using a given mrna molecule depends partly on.
You would like to create an experiment at your house. You take a sample of E.coli bacteria from the toile
to see if they will grow faster if given different samples of food. You set up 3 samples. One sample of E.coli is placed
in milk, one sample of E.coli is placed on a piece of meatloaf, and one sample of E.coli is given nutrient agar, which
simulates normal laboratory conditions. After 2 days, you count 150 colonies of E.coli in the milk, 100 colonies in the
meatloaf, and 75 on the nutrient agar.
a. Based on this information, what conclusion(s) can you draw?
b. What is your controlled variable (constant)? The same sample item.
What is your independent variable?
What is your dependent variable?
e. What group represents the controlled group?_
f. If you were to graph this experiment, what information would you put on the x-axis and why?
g. What information would you put on the y-axis? Why?
Based on this information, it shows that the E.coli bacteria grows faster in milk compared to the nutrient agar and meatloaf.
b. The controlled variable is the sample item (E.coli bacteria). The independent variable is the type of food given to the E.coli (milk, meatloaf, or nutrient agar). The dependent variable is the number of colonies of E.coli.
c. The group representing the controlled group would be the group given nutrient agar, as it represents normal laboratory conditions.
d. If you were to graph this experiment, the x-axis would represent the type of food given to the E.coli (milk, meatloaf, or nutrient agar). This is because the x-axis represents the independent variable, which is the factor being manipulated in the experiment.
e. The y-axis would represent the number of colonies of E.coli, as this is the dependent variable, which is being measured in the experiment.
What is the E.coli bacteria about?Escherichia coli, or E. coli, is a type of bacteria that is commonly found in the human intestine. It is a gram-negative, rod-shaped bacterium that is part of the normal gut microbiota, meaning that it is usually present in the human digestive tract in small numbers.
However, certain strains of E. coli can cause serious infections, particularly in people with compromised immune systems or in those who have consumed contaminated food or water.
E. coli can grow on a variety of different media, including simple sugars, complex carbohydrates, and proteins. The specific nutrients that are present in a particular growth medium can affect the rate at which E. coli grows.
Therefore, It is important to note that the growth of E. coli, like all bacteria, is influenced by a variety of factors, including temperature, pH, and the presence of oxygen.
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Which of the following organisms feeds on red blood cells?
A) Giardia lamblia
B) Escherichia coli
C) Entamoeba histolytica
D) Vibrio parahaemolyticus
E) Taenia spp.
The correct option is C ; Entamoeba histolytica Amebiasis is a parasitic infection caused by the one-celled parasite Entamoeba histolytica. Entamoeba histolytica is a protozoan that causes both intestinal and extra-intestinal amebiasis.
Although 90% of E. histolytica infections are asymptomatic, almost 50 million people become symptomatic, with around 100,000 fatalities occurring each year.
Antibiotics are required for the treatment of amoebic colitis. Metronidazole is the most often used antibiotic, however tinidazole may be a better option.
A second medication, commonly diloxanide furoate, is subsequently administered to eliminate any parasites that may still be present in your intestines.
Recovery from amebiasis is mostly determined by the severity of the symptoms. The treatment approach and prognosis are also important considerations. In moderate situations, the illness resolves in two to three days; in severe cases, it might take up to three weeks.
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trichomoniasis is a single-celled protozoan with whip-like flagella. which of the following is not a common symptom of trichomoniasis?
A common sexually transmitted ailment called trichomoniasis is brought on by a parasite. Trichomoniasis in women can result in unpleasant vaginal discharge, itchy genitalia, and painful urination.
Trichomonas in men often causes no symptoms. Trichomoniasis-infected pregnant women may be more likely to deliver their newborns early.Taking an antibiotic, such as metronidazole (Flagyl), tinidazole (Tindakan), or secnidazole, is the primary method of treatment for trichomoniasis (Solosec). All sexual partners should receive treatment at the same time to avoid contracting the infection again. By carefully using condoms each time you have sex, you can lower your risk of contracting an infection.To know more about trichomoniasis
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All of the living things in a particular ecosystem (essentially an ecosystem minus the abiotic factors)
It is true to say that all of the living things in a particular ecosystem (essentially an ecosystem minus the abiotic factors).
An ecosystem might be tiny like a patio or a lake. Or on the other hand it very well may be huge like a grassland, a desert, or a tropical jungle. There are freshwater biological systems that cover less space than saltwater environments. Notwithstanding where they are found or the way that little or enormous they might be; all biological systems are comprised of similar parts. These parts are either living or non-living parts and are mean quite a bit to the biological system.
The non-living pieces of the biological system are called abiotic factors. All living things need nonliving things to make due. A portion of these abiotic factors incorporate water, minerals, daylight, air,environment, and soil.
All living things need water to get by. Living organic entities are comprised of somewhere in the range of 50 and 95 percent water. The cycles that continue to live things alive like photosynthesis and breath can occur within the sight of water. Living things additionally need minerals like calcium, iron, phosphorus, and nitrogen. A few living things need daylight to make food. Creatures need oxygen to deliver energy for their bodies.
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Which of the following can be used to detect the location of DNA bands in a gel after electrophoresis?
SyBr green can be used to detect the location of DNA bands in a gel after electrophoresis.
What is SYBR Green I used for?
SYBR Green I is a commonly used fluorescent dye (asymmetrical cyanine dye) that binds double-stranded DNA molecules by intercalating between the DNA bases. It is used to visualise DNA in gel electrophoresis and in quantitative PCR because the fluorescence can be measured at the end of each amplification cycle to determine, relatively or absolutely, how much DNA has been amplified.
Gel electrophoresis is a method used to separate mixtures of DNA, RNA, or proteins according to molecular size. In it, the molecules to be separated are pushed by an electrical field through a gel that contains small pores. When an electric current is applied to the gel, DNA will migrate towards the positively charged electrode because DNA is negatively charged. Shorter strands of DNA move more quickly through the gel than longer strands resulting in the fragments being arranged in order of size.
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Which one of these is a sign of a weak economy?
The sign of weak economy in a country is that there would be rising inflation rates.
What is a weak economy?A weak economy is defined as the type of financial situation that occurs when there is weak to negligible growth of the financial state of a country.
The characteristics of a weak economy include the following:
Increase in the rate of unemployment: With high jobless numbers, consumers will lose their purchasing power and eventually lower demand.Rising inflation in the society: This is an unevenly rising prices which inevitably reduces the purchasing power of some consumers.Declining property sales: When there is decrease in sales of property, this means that there is impending economic depression.Increasing credit card debt defaults: A rise in debt default means that people are losing their ability to pay, which signals an economic depression.Learn more about financial economy here:
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The structure of a protein dietates the partners with which it can interact. Therefore, the structure of a protein is directly related to its The contours of a protein determine the that its interaction partner must have, whereas the surface chemistry of a protein determines the kinds of chemical interactions that the protein will make with its interaction partner (e.g. Coulombic interactions or) Therefore, a protein will only bind to molecules that have the appropriate shape and chemistry (i.e. only those that are the protein). Biochemists talk about protein structure at four distinct levels: primary, secondary, tertiary and quaternary structure. Below are depictions of each of these levels of protein structure. For each image, match the term and the written description of the level of protein structure that the image depicts. Drag the appropriate items to their respective bins.
Proteins are the polymers of amino acids. Amino acids are joined together by peptide bonds to make long chains of polypeptides.
What is cell membrane function?Both a hydrophilic and a hydrophobic area exist in phospholipids. A fluid structure, the membrane is made up of a mosaic of different proteins that are either linked to or embedded in a double layer of phospholipids.All cells have a cellular membrane, also known as a cell surface, which divides the cell as from external environment. A semipermeable basement membrane makes up the cell membrane. The movement of molecules into and out of the cell is controlled by the cellular membranes.Thin shells to form complete boundaries within cells are called membranes. Lipids, peptides, and carbohydrate make up the components of cell membranes. A mucous membrane makes up cell membranes. Non-covalent forces hold together cellular membranes. The structure of walls is fluid-like.To learn more about Coulombic interactions refer to:
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Can I get help with this review for Oceanography please?
According to your textbook and many other sources of information, the shortest phase of mitosis is anaphase. Prophase takes 30 to 60 minutes; metaphase, 2 to 10 minutes; anaphase 2 to 3 minutes; telophase 3 to 12 minutes.
In the mitosis lab that you have just completed, the shortest phase was telophase and/or metaphase. Please discuss these results. Why do you think our results do not align with your textbook or other sources of information? Why does not anaphase come out as the shortest mitotic phase in our experiment?
Anaphase does not appear as the shortest mitotic phase in our experiment because the conditions must not be right because anaphase is the shortest phase of mitosis, it starts abruptly with the simultaneous separation of sister chromatids and is completed within few minutes. In this step, each chromatid begins to move toward the opposite side of the cell.
What is the function of mitosis?Mitosis is a process of cell division that is of great importance to organisms. In multicellular beings, mitosis is important to ensure the growth of these individuals and also for tissue regeneration. In unicellular organisms, mitosis has the important function of ensuring asexual reproduction.
What are the stages of mitosis?Mitosis consists of four basic phases: prophase, metaphase, anaphase, telophase. Some textbooks list as many as five, dividing prophase into an earlier phase (called prophase) and a later phase (called prometaphase).
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The mechanism that establishes the medullary osmotic gradient depends most on the permeability properties of the ________.nephron loop
The mechanism that establishes the medullary osmotic gradient depends most on the permeability properties of the loop of Henle.
In general , osmotic gradient is rooted and maintained by selective permeability in the loops of Henle also termed as countercurrent multipliers and passive diffusion in the vasa recta also termed as countercurrent exchangers.
Normally , Cortical and juxtamedullary nephrons are responsible for maintaining the absorption of solutes and water in the blood, Also the countercurrent multiplication in the loops of Henle of juxtamedullary nephrons is largely responsible for developing the osmotic gradients that are responsible for concentrating the urine.
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5. An agonist is a chemical capable of binding to a receptor and initiating a reaction. An
antagonist is a chemical that binds to the receptor but does not cause a reaction, effectively
blocking that receptor. Sketch a model (using the one you colored as a guide) to show how an
antagonist works.
An antagonist is a substance that binds to the receptor either at the primary site or at a different site and prevents the receptor from responding.
What is the Antagonist mechanism?The opposite of what an agonist does is an antagonist. It attaches to receptors and prevents the receptor from eliciting the desired effect. Reverting to the analogy, it would be like plugging the coin slot of the machine, preventing it from operating until the obstruction is cleared.
What are receptors?
Receptors are characterized as specific cell membrane structures. They are mostly made up of proteins that bind to ligands and trigger reactions. There are hundreds of distinct G-protein-linked receptors. The adrenergic receptor, the muscarinic type of acetylcholine receptor, metabotropic glutamate receptors, odorant receptors in the olfactory system, and several forms of peptide hormone receptors.
Hence, an antagonist is a substance that binds to the receptor either at the primary site or at a different site and prevents the receptor from responding.
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Why is heredity so important to cells?
Explanation:
Perhaps the most fundamental property of all living things is the ability to reproduce. All organisms inherit the genetic information specifying their structure and function from their parents. Likewise, all cells arise from preexisting cells, so the genetic material must be replicated and passed from parent to progeny cell at each cell division. How genetic information is replicated and transmitted from cell to cell and organism to organism thus represents a question that is central to all of biology? Consequently, elucidation of the mechanisms of genetic transmission and identification of the genetic material as DNA were discoveries that formed the foundation of our current understanding of biology at the molecular level.
Heredity is important to all living organisms as it determines which traits are passed from parent to child. Successful traits are more frequently passed along and over time can change a species. Changes in traits can allow organisms to adapt to specific environments for better rates of survival.
9. One of Opal's children is born with shocking red hair. Is Orville the father of this child But wait, Opal swears she has been faithful and claims that the hospital
goofed and got her baby mixed with another. Is this a plausible explanation?
No this is not a plausible explanation. All four of your grandparents' genetic material came together to create you. However, that does not necessarily imply that you receive an equal contribution from each one of them.
Can grandparents pass on genetic traits to their grandkids?
However, on average, each grandmother contributes around 25% of your genetic makeup. Yes, a child's appearance may be influenced by their grandparents' genes. After all, each of a grandchild's grandparents contributes 25% of their genetic makeup. Additionally, genes include the instructions for how we seem (and most everything else about us).
Actually, you have more genes from your mother than from your father. This is due to the mitochondria, which are tiny organelles found inside your cells and which you can only get from your mother.
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Being born with extra fingers on a hand is called polydactyly. This is more common in some populations in North America than others due to ____.
A. an increase in mutation rate in these populations
B. random mating with other populations in North America
C. migration of people with multiple fingers out of the population
D. a founder effect because their ancestors from Europe carried the alleles
E. natural selection in favor of extra fingers in these populations
D. a founder effect because their ancestors from Europe carried the alleles.From one generation to the next, the frequency of alleles (genetic variations) will remain constant.
What does a population need to do to achieve Hardy-Weinberg equilibrium?According to the Hardy-Weinberg equilibrium, if favorable influences exist, genetic variation in a population will remain stable from one generation to the next.
What causes population genetic drift?Genetic drift is a random process that can produce significant population changes rapidly . Recurringly small population sizes, drastic population size reductions known as "bottlenecks," and founder events, in which a new population is created from a small number of people, all contribute to random drift.
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Dna replication escape room level 2
The enzyme that unwinds the double helix of DNA in preparation for DNA replication is called RNA helicase.
What is DNA replication ?A double-stranded DNA molecule is copied to create two identical DNA molecules through the process of DNA replication. Because every time a cell splits, the two new daughter cells must have the same genetic material, or DNA, as the parent cell, replication is a crucial process. DNA replication, as used in molecular biology, is the biological process that creates two identical copies of DNA from a single original DNA molecule. All living things replicate their DNA, which is the primary mechanism for biological heredity.
The series of events that occur during prokaryotic DNA replication have been explained below.
InitiationPrimer SynthesisLeading Strand SynthesisLagging Strand SynthesisPrimer RemovalLigationTermination.To know more about DNA replication please click here ; https://brainly.com/question/21265857
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NOTE: The given question is incomplete on the portal. Here is the complete question.
QUESTION: The enzyme that unwinds the double helix of DNA in preparation for DNA replication is called
match the following environmental conditions to the level of transcription of the lac operon that would occur under those conditions
Only in the absence of glucose can the lac operon be highly expressed. This method makes sure that bacteria only activate the lac operon and begin utilizing lactose after exhausting all of their preferred energy source (glucose).
How does the lac operon become transcriptionally active?One: The lac Operon The lac operon's transcription is tightly regulated so that expression only happens when lactose is available to act as an alternative fuel source and glucose is insufficient.
What circumstances result in the highest level of transcription of the structural genes in the lac operon?When lactose is available, transcription of the lac operon genes takes place, resulting in the production of the inducer and subsequent silencing of the repressor.
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Minha's mother wants to buy curtains for all the windows of their house. She asked Minha to measure the length of the existing curtains which serve as a ready reference while buying the new ones. If an elastic measuring tape is used for measuring the length of the curtains, will Minha get an accurate measurement? Why? Give reason.
If an elastic measuring tape is used for measuring the length of the curtains, will Minha not get an accurate measurement. The length will be reduced. This is due to elasticity.
What is elasticity?This refers to the ability of an object/ material to return to its original shape/length after a stretch.
When an elastic tape is used in the measurement, the length gotten is likely to be inaccurate as the tape will be stretched during the measurement and return to its original length after measurement giving a shortened length.
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Two amino acids you need to form an
Answer:
Dipeptide
Explanation:
The two joined amino acids are called a dipeptide. The amide bond is synthesized when the carboxyl group of one amino acid molecule reacts with the amino group of the other amino acid molecule, causing the release of a molecule of water (H2O), hence the process is a dehydration synthesis reaction.
An apple, potato, and onion all taste the same if you eat them with your nose plugged
WHY IS THIS
Answer:without your nose you taste
Explanation:
use book
This is because most of what we perceive as “taste” is due to our sense of smell. Our brain combines the information from both scent and taste to create the overall flavor of food or drink. When we plug our nose, we block our sense of smell and impair the connection between our nasal cavity and brain. This makes it hard to identify the flavor of food, which consists of smell, taste, temperature, texture, spiciness, and so on. Therefore, apples potatoes and onions may taste similar if we eat them with our noses plugged.
Individuals with mutations in excision repair enzymes may suffer from ____ due to unrepaired DNA damage caused by ____. skin cancer; the sun's UV rays.
Skin cancer may develop in people who have mutations in the excision repair enzymes as a result of unrepaired DNA damage brought on by the sun's UV radiation.
Excision repair involves identifying the damaged DNA and removing it, either as single bases or even as nucleotides. A new DNA strand is subsequently created, using the unharmed complimentary strand as a template, to close the resultant gap. DNA ligase, a specialized enzyme, surrounds a double helix to fix a damaged strand of DNA (seen here in color). The billions of DNA breaks that occur naturally over a cell's life are repaired by DNA ligase. Moreover, errors start to accumulate much more quickly when the alleles for the Dna synthesis enzymes itself are altered. Such mutations can cause cancer in eukaryotes.
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Which Of The Following Demonstrates Population Growth When There Is Limited Food In An Area?
Answer:
C
Because number of individuals are low so less population growth due to less food babies probably die
Which of the following would be the most likely to enhance the strength of smooth muscle contraction upon stimulation?
A. A decrease in calmodulin concentration. B. An increase in phospholipase C activity
C. An increase in sarcoplasmic reticulum Catt- ATPase activity.
The most likely way to strengthen the contraction of smooth muscle after stimulation is by an increase in phospholipase.
The correct answer is B
What sort of excitement would that be?External or internal stimuli are both possible. Your body's reaction to a medication is an illustration of an external stimulus. Your vital signs changing because of a change in the body is an illustration of an internal stimulus.
What does stimulation feel like?Traditionally, when body stimulation is felt, it is referred to as a feeling, such as a type of touch, a taste or a smell, or even a painful or enjoyable experience. This might be viewed as mental stimulation, a type of stimulus that has an impact on a person's mental or feeling processes.
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Generally, the product life cycle consists of five stages; development, introduction, growth, maturity, and decline.a. trueb. false
Generally, the product life cycle consists of five stages; development, introduction, growth, maturity, and decline. a. true
What is the average product life cycle?
The product life cycle describes the steps that a product goes through from the moment it is launched to the market until it is removed from the market. A product's life cycle is divided into four stages: introduction, growth, maturity, and decline. Many items are still in their early stages of development.
The life cycle of a product is often divided into four stages: introduction, growth, maturity, and decline. Management and marketing experts utilise product life cycles to assist establish advertising schedules, pricing points, growth into new product markets, package redesigns, and other factors.
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An increase in blood levels of carbon dioxide results in a decrease in blood pH because an increase in PCO2
a) none of these answers is correct.
b) decreases H+ ion levels in the plasma.
c) reacts with water to form carbonic acid, which dissociates to release H+.
d) promotes an increase in lactic acid production in the muscle.
An increase in blood levels of carbon dioxide directly affects blood pH by promoting the formation of carbonic acid, which releases H⁺ ions and lowers the pH of the blood. The correct option is c.
When carbon dioxide enters the bloodstream, it can react with water (H₂O) to form carbonic acid (H₂CO₃) through the action of an enzyme called carbonic anhydrase. Carbonic acid is a weak acid that can dissociate, releasing hydrogen ions (H⁺).
The equation for this reaction is:
CO₂ + H₂O ⇌ H₂CO₃ ⇌ H² + HCO₃⁻
The increase in blood levels of carbon dioxide leads to an increase in PCO₂ (partial pressure of carbon dioxide). This elevated PCO₂ drives the reaction towards the right, favoring the formation of carbonic acid and resulting in an increase in H⁺ ions. The accumulation of H⁺ ions in the blood decreases blood pH, making it more acidic.
Thus, the correct option is c.
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What happens to the cotyledons of pea and maize seeds?
A) They surround the base of the shoot to provide additional strength.
B) They form a protective sheath around the developing root system.
C) They remain in the soil and decompose.
D) They immediately begin to use the sun's energy in photosynthesis.
Cotyledons of pea and maize seeds is B) They form a protective sheath around the developing root system.So, correct option is B.
A seed is a significant piece of a blooming plant, which leads to another plant on seed germination. The seeds fluctuate in their shapes, varieties and sizes. A few seeds are oval in shape, while some are round, crumpled, shaggy, or winged. The principal parts of a seed are:
Incipient organismSeed CoatEndospermThe various pieces of an undeveloped organism are:
RadicleEpicotylHypocotylCotyledonsCotyledon alludes to the huge piece of the undeveloped organism tracked down inside the seed. The first and the delicate leaf arises when the seed develops. It is likewise called the capacity unit of a seed, as it gives supplements to the various pieces of an incipient organism.
Pea and maize seeds forms a protective sheath around the root so that it cannot be effected by the external environmental factors and develops smoothly without any hindrance.Root development is favorable for plant development too.
Hence,option B is correct.
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Place the following statements that describe the 5 steps in an adaptive immune response in the order in which they occur.
A. Long lived-immune cells allow the second response to a given microbe to be stronger and faster than the first response
B. Adaptive immune cells are born in the bone marrow, then mature in either the bone marrow or the thymus
C. Adaptive immune cells use specific receptor proteins to bind to antigens very specifically
D. Intracellular signaling pathways are initiated, causing the adaptive immune cell to change cell division, gene expression, and protein translation
E. Adaptive immune cells kill invading microbes, either directly or indirectly
In B cells, Helper T cells, and Cytotoxic T cells, the adaptive immune response took place over the course of four phases: encounter, activation, attack, and memory.
What is the sequential order of the immunological response?The stages of the development of all adaptive immune responses are: antigen recognition; activation of particular lymphocytes to promote proliferation and differentiate into effector and memory cells; removal of the antigen; and decline of the response, with memory cells being the long-lived survivors.
What happens during an adaptive immune response, according to this quiz?Antigens are present on all external invaders of the body and can trigger the adaptive immune response. In order to recognize, neutralize, and eliminate the infections, the adaptive immune response might then create antibodies that bind to the antigens.
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what's the difference between limiting factor and carrying capacity
Limiting factors and carrying capacity are connected to the number of organisms in an ecosystem.
Limiting Factor
A limiting factor is any part of an environment that controls a population within one area. For example, available food is a limiting factor. There is not unlimited food, so it limits how many organisms can survive in one area. Organisms can only survive if there is enough food for them. Space is also a limiting factor because there is only so much space for the population. Overall, any factor that forces the organisms to compete or that limits resources is a limiting factor.
Carrying Capacity
The carrying capacity of an area is the total number of organisms that an ecosystem can safely support. For example, one ecosystem might have a carrying capacity of 5,000 bunnies. This means that the ecosystem cannot sustain more than 5,000 bunnies. When a population begins to near the carrying capacity, birth rate will decrease or death rate will increase. Since resources start to dwindle as carrying capacity is reached, it becomes more difficult for the population to grow.
The limiting factors of an ecosystem create the carrying capacity. For instance, if there is only enough food to feed 5,000 bunnies, then the carrying capacity will be 5,000.