micro which of the following methods of action would be bacteriostatic? a) competitive inhibition with folic acid synthesis b) inhibition of protein synthesis c) injury to plasma membrane d) inhibition of cell wall synthesis e) competitive inhibition with dna gyrase

Answers

Answer 1

Bacteriostatic action would be inhibited protein synthesis.

A bacteriostatic agent, also known as a bacteriostat, is a biological or chemical agent that prevents bacteria from reproducing while not necessarily killing them. Bacteriostatic antibiotics, disinfectants, antiseptics, and preservatives can be distinguished based on their application. Bacteriocidal methods include heat, filtration, radiation, and chemical exposure. Heat sterilisation is a widely used method in microbiology laboratories.

Microorganisms such as bacteria, fungi, and yeast are incinerated by the dry heat of an open flame. Tetracyclines and gly-cylcyclines, chloramphenicol, macrolides and ketolides, lincosamides, streptogramins ,oxazolidinones, and aminocyclitols are bacteriostatic protein-synthesis inhibitors that target the ribosome. Bacteriostatic antibiotics inhibit bacterial growth by interfering with protein production, DNA replication, and other aspects of bacterial cellular metabolism.

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Related Questions

Explain how radiometric dating is used to determine the age of an object.
Be sure to give details.

Answers

Radiometric dating, which examines the ratio of two distinct isotopes in a sample, establishes the age of rocks.

How does radiometric dating work to establish an object's age?

The fundamental idea behind radiometric dating is that by comparing an isotope's presence in a sample to its abundance on Earth and its known half-life (rate of decay), you may determine the sample's age.

What can we determine using radiometric dating?

It offers an important source of knowledge regarding the ages of fossils and the estimated rates of evolutionary change by enabling the development of geological timelines.

Ancient artefacts and archaeological materials are both dated using radiometric methods

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chemicals called uncouplers can make membranes permeable to small molecules and ions. what effect might uncouplers have on cellular respiration?

Answers

Uncouplers may have an adverse effect on cellular respiration, resulting in lower ATP synthesis since the proton gradient would be reduced.

How could uncouplers affect cellular respiration?

Increasing the amount of cellular respiration is possible by including an uncoupler (FCCP), which reduces the proton motive force by causing a short circuit of protons on the inner mitochondrial membrane. Substrate oxidation in this condition serves as the primary predictor of oxygen consumption.

How do uncouplers impact the cycle of electron transport?

Uncouplers of oxidative phosphorylation prevent the electron transport and phosphorylation processes from coupling, which prevents the production of ATP without disrupting the respiratory chain or ATP synthase (H(+)-ATPase).

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Typically, a lymphocyte will encounter its first exposure to a foreign antigen in the:
Red bone marrow
Yellow bone marrow
Thymus gland
Secondary lymphatic structures

Answers

A lymphocyte will encounter its first exposure to a foreign antigen in the Red bone marrow.

What does having low lymphocytes mean?These cells aid in defending your body against illness. You are more vulnerable to infection if you have an inadequate amount of lymphocytes (lymphopenia). Lymphopenia symptoms vary in severity depending on how severe the condition is and how long it has persisted. Some individuals show no symptoms.Most animals have lymphocytes as a sort of white blood cell in their immune systems. Natural killer cells, T cells, and B cells are examples of lymphocytes. They are the primary class of cells in lymph, thus the term "lymphocyte." Between 18% and 42% of the white blood cells that are in circulation are lymphocytes.

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What creates the boundaries for the distribution of a trait within a population?

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Environment creates the boundaries for the distribution of a trait within a population.

Environmental factors can also cause gene mutations, and the most viable mutations are those that can be passed down across generations. Environmental factors also have an impact on how traits manifest, which has an impact on the probability that a trait will emerge in a population. Gene expression can be changed by environmental circumstances, which can affect traits that are determined by heredity. Natural selection, a crucial evolutionary mechanism, is a slow, long-term shift in the genetic composition of populations.

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What are the common types of mutations that occur to convert proto-oncogenes to oncogenes?.

Answers

Amplifications and Hypermethylation of promoter sequences are the common types of mutations that occur to convert proto-oncogenes to oncogenes.

A proto-oncogene is a normal gene that is present in a cell. Numerous proto-oncogenes exist. Each one is in charge of producing a protein essential for cell division, growth, and other functions. These genes often carry out their functions without incident.

However, if a proto-oncogene makes a mistake (mutates), the gene may turn on when it shouldn't. If this occurs, the proto-oncogene may develop into an oncogene, a gene that causes cancer. Cancer develops as a result of uncontrolled cell growth.

A collection of typical genes in a cell make up proto-oncogenes.They contain the data required by your body to create the proteins in charge of encouraging cell division, which allows for cell development, suppressing apoptosis, also known as cell death, which is the process by which cells change their role by inhibiting cell differentiation, For cells to maintain healthy tissues and organs in your body, these processes are all necessary.

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corona viruses are approximately 0.5micm in diameter how many coronaviruses could fir across a water froplet that was 1cm across

Answers

Number of corona virus that can be fitted into one water droplet is 39990

The volume of a sphere is the amount of space occupied inside the sphere. A sphere is defined as a three-dimensional round solid with all points on the sphere equidistant from the center. The fixed distance is called the radius of the sphere and the fixed point is called the center of the sphere.

Explanation:

1 mm is equals to 1 x 10-4 cm

Given, the diameter of corona virus is 0.5 mm i.e equals to 5 x 10-5 cm

Assuming corona virus is spherical in shape,  volume of one corona virus is

                         Volume(c)=[tex]\frac{4}{3} \pi r^{3}[/tex]

Where, r is the radius of virus = diameter of virus/2

                          = 5 x 10-5 / 2 = 2.5 x 10-5

Putting value of r in above equation,

                 Volume(c)=[tex]\frac{4}{3} \pi (2.5*10^{-5}) ^{3}[/tex]

                                  = 0.0001047 cm3

Now we have to calculate volume of one water droplet. We have diameter of water droplet is 1 cm . So volume will be

              Volume(w)=[tex]\frac{4}{3} \pi (1) ^{3}[/tex]

                                =  4.187 cm3

Number of corona virus that can be fitted into one water droplet is Volume of one water droplet / volume of one corona virus  = Volume (W) / Volume (C)  = 4.187/ 0.0001047 = 39990.448 = 39990

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Which of these factors does cites fail to consider when determining which species to cover?.

Answers

The sustainability of the Ecosystem is the factor that CITES neglects to take into account when choosing which species to cover. A biological setting with several habitats that are capable of thriving and supporting itself independently is referred to as a sustainable ecosystem.

The biosphere can meet the needs of the current generation without compromising the ability of future generations to meet their own needs.

Sustainability is essential for many reasons, including Environmental Quality - Healthy communities require toxic-free environments, clean air, and natural resources. Growth - As UNTHSC's enrollment keeps expanding, we need additional space, energy, and water.

Thus, we can conclude that CITES fails to consider the sustainability of the ecosystem when deciding which species to include in its list of protected species.

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Your question is incomplete. Please find the complete question below.

Which of these factors do CITES fail to consider when determining which species to cover?

a.Species population

b.Economic impact

c.Analysis of trade

d.Ecosystem sustainability

Pathogenic bacteria are likely to multiply rapidly when environmental temperatures are between.

Answers

Answer:

20-40

Explanation:

they multiply in an environment at lower temperature

Two inect group vary genetically by two gene. When put together, they were able to reproduce and produce offpring that could alo breed. Which bet decribe thi inect population?

Answers

They are the same species of insects.

A group of organisms that can reproduce sexually and can result in live, fertile offspring is referred to be a species. Even though there are two genes that separate two insect groups genetically, if they are compatible for mating, they can produce fertile offspring, especially if they are of the same species.

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If a color-blind man marries a woman who is a carrier for color blindness, it is most probable that

A. all of their sons will have normal color vision.
B. half of their sons will be color-blind.
C. all of their sons will be color-blind.
D. none of their children will have normal color vision.


The answer is C right?

Answers

Answer:

B. half of their sons will be color-blind.

Explanation:

If a color-blind man marries a woman who is a carrier for color blindness, their children will inherit one X chromosome from the mother and one from the father. If a son inherits the abnormal X chromosome from the mother, he will be color-blind. Since the mother has one normal X chromosome and one abnormal X chromosome, there is a 50% chance that a son will inherit the abnormal X chromosome and be color-blind.

human hair type is transmitted as an (autosomal) incomplete dominant trait. if curly-haired paul marries straight-haired pam, what percent of their children would have curly hair?

Answers

Human hair type is transmitted as an (autosomal) incomplete dominant trait, so if curly-haired Paul marries straight-haired pam, percent of their children that would have curly hair is 0%.

What is an autosomal incomplete dominance?

Incomplete dominance is a form of gene interaction where both alleles of gene at a locus are partially expressed, resulting in an intermediate or different phenotype. It is also called partial dominance.

This pattern of inheritance is described as incomplete dominance because neither of the alleles is completely dominant over the other as both alleles can be seen at the same time. For example: allele for red flowers is incompletely dominant over the allele for white flowers, so Red + white = pink.

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Some plants have stems called that grow along the ground, while fungi have hyphae that grow underground. These plant stems are involved in and hyphae are involved in. Both of these structures help the organisms.

Answers

Answer:

1. Runners2. Nutrient distribution3. Nutrient absorption 4. Reproduce

Explanation:

Answer:

As the guy above said, 1: runners, 2: nutrient distribution, 3: nutrient absorption, and 4: reproduce.

Hope this helps!

Explanation:

What are the common types of mutations that occur to convert proto-oncogenes to oncogenes?.

Answers

Answer:

Point mutation. This mutation alters, inserts, or deletes one or more nucleotides (building blocks of DNA and RNA) in a gene sequence. ...

Gene amplification.

Chromosomal translocation (rearrangement).

8. Which of the following best represents the energy flow
between photosynthesis and
cellular respiration?

a. Sunlight → photosynthesis →→ ATP → cellular
respiration →→ Glucose
-
b. Sunlight → cellular respiration → ATP →
photosynthesis → ADP

c. Sunlight → photosynthesis →→ Glucose → cellular
respiration →→ ATP

d. Sunlight → cellular respiration→ Glucose →
photosynthesis →→ATP
OD

Answers

carbohydrates, cellular respiration, ATP, light, photosynthesis, and water best represents the energy flow between photosynthesis and cellular respiration

Plants (including algae and some bacteria) employ sunlight, water, and CO2 during photosynthesis to produce sugars (carbohydrates). This reaction, which takes place in chloroplasts, results in the reduction of CO2 (gaining electrons), oxidation of water (losing electrons), and production of oxygen (O2). The usage of that O2 in cellular respiration follows. Cellular respiration takes place in mitochondria and consumes glucose and oxygen to create ATP, which is the energy source. H2O and CO2 are created.

Photosynthesis is essential to most life on Earth. Plants, microalgae, as well as some types of bacteria carry out the process by capturing solar energy to create oxygen (O2) plus biochemical power is supplied in glucose (a sugar). Consequently, herbivores get this energy from consuming plants, and carnivores get it from eating herbivores.

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How are these limbs different​

Answers

Answer:

They all have different functions and different methods of transportation for each of the following limbs.

Where is the center of the anatomic origin of the anterior talofibular ligament on the fibula?.

Answers

Answer:

Hope this helps!

Explanation:

The fibular origins of ATFL and CFL are concentrated in the lower part of the lateral malleolus and connected with connective fiber [18]. Also, ligament injury is likely to occur at the fibular side or substantial part of the ligament [4, 6]

the following figure shows the general steps that occur when a researcher uses the crispr-cas9 system to modify a protein-encoding gene in a eukaryotic cell with the goal of modifying the protein product. drag the descriptions of the steps to their appropriate locations on the figure.

Answers

A scientific advance has been made thanks to CRISPR-Cas9 editing. Many scientists are interested in this and have successfully edited DNA using this method.

Although the use of guide RNA is not new, it has made great strides in directing caspases toward their target sequences.Although the mutations have been reported, they cannot yet be verified. Although it has been reported that CRISPR-Cas causes single base pair deletions in DNA far from its activity site, which lead to gene impairment, the processes of DNA alterations through CRISPR-Cas have not yet established any form of reliable approach.After CRISPR was used to edit DNA, significant changes were seen in DNA sequences thousands of bases away from the region of action.

However, there is currently no experimental data that reveals the specific mechanism.

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Which of the following does not serve as a way to neutralize the charge in a body? Question 18 options: A) Bringing the charged body into contact with another body having an equal but opposite charge B) Adding free electrons to a positively charged body C) Adding more protons to a positively charged body until the number of protons matches the number of electrons D) Allowing free electrons to escape from a negatively charged body

Answers

Adding free electrons to a positively charged body does not serve as a way to neutralize the charge in a body. Option B is correct.

How do you neutralize a charge?

Static electricity can be effectively eliminated by grounding a conductive or dissipative conductor. A non-conducting insulator holds an electronic charge and cannot be grounded to "drain" the charge.

All surfaces, products and personnel are electrically grounded to ground all conductors according to the first ESD control principle. Bonding means connecting or connecting through a resistor, typically 1 to 10 megohms.

A wristband dissipates charge from the operator and a properly grounded mat provides a path to ground for his exposed ESD sensitive equipment.

Movable objects (containers, tools, etc.) are glued by standing on the glued surface or held by the person being glued.

If the static charge in question is on something that cannot be grounded. B. With insulators, ESD control principle number 2 applies and the insulators must be removed. According to the ANSI/ESD S20.20 ESD standard, "All non-essential insulation such as coffee cups, food wraps, and personal items must be removed from the EPA."

Therefore, Adding free electrons to a positively charged body does not serve as a way to neutralize the charge in a body. Option B is correct.

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which of the following most accurately describes biomass energy? select one: a. energy produced with nuclear atoms are split. b. energy produced from burning recently living organisms. c. energy produced from heat stored deep within the earth. d. energy produced from burning ancient, fossilized remains of organisms.

Answers

Option B. Energy produced from burning recently living organisms, most accurately describes biomass energy.

Energy created or generated by living or formerly living organismsis known as biomass energy. Plants like the aforementioned corn and soy are the most prevalent types of  biomass energy that are utilized for energy. These organisms energy may be used to generate electricity or be burnt to provide heat.  biomass energy . When organic material is burnt as a fuel source, electricity can be produced.  biomass energy is the term for these fuels, which can be anything from plants to wood to organisms . Crop wastes, for example, are a source of biomass, a sustainable energy source made from organisms ,forest waste. Purpose-grown grasses.

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Water lilies do not grow in desert sand because water availability to these plants in a desert is.

Answers

Water supply to these plants in a desert is a limiting element, hence water lilies cannot flourish in desert sand.

A perennial aquatic plant with floating leaves known as a fragrant water lily is often found in water that is 3 to 6 feet deep. It grows in ditches, slow-moving streams, freshwater lakes, and ponds. It is pH-tolerant across a wide range. prefers muddy lake/pond bottoms to silty ones.

The growth of water lilies requires sunlight. While they can tolerate moderate shadow for a while, they still require sunlight for at least three hours each day. You should be careful to place them far from any pumps or leisure areas because moving water is harmful to their growth.

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the duodenum contains these structures whose products neutralize the acidic material from the stomach.

Answers

The duodenum and stomach are divided by the pyloric sphincter. Pancreatic fluids, an alkaline mixture rich in bicarbonate, are mixed with stomach chyme to balance its acidity.

What part in the body does the duodenum play?

The small intestine begins in the duodenum. The major function of the duodenum is to complete the initial stages of digestion. Food from the stomach is combined with bile from the gallbladder and other substances in this area of the intestine.

What issues could develop in the duodenum?

Dyspepsia or epigastric discomfort, anorexia, obstructive symptoms, early satiety, nausea, vomit, & weight loss are all symptoms that patients with duodenal lesions exhibit. Long-term sickness raises the chance of duodenal cancer, which can manifest as inflammatory lesions, prohibitions, and fistulas.

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Adding or removing a single atom will turn a molecule into a molecule of a different substance. How is combining atoms to form a molecule like mixing ingredients for a recipe? pick the correct option.

Answers

Adding or removing a single atom can turn a molecule into a molecule of a different substance, just as adding or removing a single ingredient can change the nature of a recipe. In both cases, the combination of atoms or ingredients determines the characteristics of the final product, such as its chemical or physical properties, taste, or texture.

Just as a recipe specifies the quantities and types of ingredients that should be combined to produce a desired dish, the chemical formula of a molecule specifies the quantities and types of atoms that should be combined to produce a particular molecule. For example, the chemical formula for water, H2O, indicates that two hydrogen atoms should be combined with one oxygen atom to produce a molecule of water.

In the same way that a chef must carefully measure and mix the ingredients in a recipe to produce the desired outcome, a chemist must carefully control the quantities and types of atoms that are combined to produce a particular molecule. Changing the quantities or types of atoms in a molecule, or the quantities or types of ingredients in a recipe, can alter the properties of the final product.

Overall, combining atoms to form a molecule is similar to mixing ingredients for a recipe in that both processes involve the careful combination of specific quantities and types of components to produce a desired outcome.

Construct a food chain. Label the producer, primary consumer, secondary consumer, and tertiary consumer. An owl eats a snake, the snake eats a squirrel, the squirrel ate a nut.

Answers

Answer:

Owl - tertiary consumer

⬆️

Snake - secondary consumer

⬆️

Squirrel - primary consumer

⬆️

Nut - producer

what are examples of types of chemical markers associated with dna that determines when, where and by how much genes are expressed in each cell?

Answers

Histone proteins and epigenetic factors work together to determine when, where and at what level genes are expressed in each cell.

How many genetic markers are used to identify a human?

Most of the methodological approaches to identify an individual are based on two types of molecular genetic markers: 1)  STRs (Short tandem repeats and 2) SNPs (single nucleotide polymorphisms).

What chemicals affect gene expression?

In vitro, animal, and human studies have found different classes of environmental chemicals that alter epigenetic marks, including metals (cadmium, arsenic, nickel, chromium, methylmercury), air pollutants and peroxisome proliferators (trichlorethylene, dichloroacetic acid, trichloroacetic acid).

How many markers are there in DNA?

The advanced results up to 68 DNA markers, compared to the industry standard 16 DNA markers, with a result accuracy of 99.9999%. Testing fewer markers may seem cheaper, but clients are more likely to be inconclusive about all significant results

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in research with nonhuman primates, which term refers to feelings of concern for others stemming from understanding their feelings?

Answers

Anthropomorphism is the feelings of concern for others stemming from understanding their feelings.

Anthropomorphism is when non-human entities are given human characteristics, feelings, or intentions. It is said to be a built-in psychological predisposition in people.The practise of attributing human traits or behaviours to non-human beings, especially animals, is known as anthropomorphism. It is a typical way of viewing and interacting with the environment, however some people are more attracted to it than others.Personification, a similar term, is the attribution of human shape and traits to abstract notions like nations, feelings, and physical phenomena like the seasons and weather.Both have long histories as literary and artistic methods, and traditional fables with anthropomorphized animals are found in most civilizations. Both domesticated and wild animals have frequently been given human emotions and behavioral characteristics.

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How does a negative feedback
loop maintain homeostasis?
A. it creates an external response
B. it works to bring the body back to the target of
stability
C. it moves the characteristic above normal
limits
D. it creates an increase in product of a reaction

Answers

Negative feedback helps to keep a variable within its regular range and lessens an overreaction.

How does unfavourable criticism work and what is it?

Negative feedback occurs when an output of a system, process, or mechanism is given back in a way that aims to reduce oscillations in the output, whether these oscillations are caused by changes in the input or other disturbances.

What possible repercussions could negative feedback have?

Because the feedback energy (voltage or current) is out of phase with the input signal when there is negative feedback, the input signal opposes the feedback energy. Negative feedback reduces the amplifier's gain. It also reduces distortion, noise, and instability. The bandwidth is increased while the input and output impedances are improved by this feedback.

Become familiar with Bandwidth.

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Answer: B

Explanation:

Genetic variation among humans is relatively small when compared to other species. Where in the human genome does most of the diversity occur?.

Answers

The genetic variations seen in and between populations are known as human genetic variation.

Humans differ genetically from one another. Due to developmental mutations and gene copy-number variation, even monozygotic twins, who arise from a single zygote, may differ genetically. Techniques like genetic fingerprinting rely on individual differences, even between closely related people.

There are 324 million identified variations from sequenced human genomes as of 2017.

A person's genome typically differs from the reference genome by 20 million base pairs, or 0.6% of the total 3.2 billion base pairs, as of 2015.

In contrast to other species, humans are genetically uniform. While a limited number of genetic variations are found more commonly in particular geographic regions or in individuals with ancestry from those places, this variance accounts for the majority of genetic variation.

Genetic variation between populations occurs for at least three reasons. If an allele offers a competitive advantage, natural selection may endow people with an adaptive advantage in a particular environment.

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A blood platelet drifts along with the flow of blood through an artery that is partially blocked by deposits. As the platelet moves from the narrow region to the wider region, it experiences.

Answers

Hola hola cómo te sientes de que me ha dado la vuelta de vuelta a la vuelta

Explain how cells work together to create an organ system.

Answers

Cells work together to create an organ system through the process of differentiation.

What is a Cell?

This is referred to as the smallest or simplest unit of life and they work together to form tissues, tissues work together to form organs, and organs work together to form organ systems in the body of organisms.

The process of cell differentiation allows multi-cellular organisms to create uniquely functional cell types and body plans and an example is the cardiac muscle cells which group together to form cardiac muscle tissue, which in turn forms the heart and is therefore the ways in which  cells work together to create an organ system.

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considering how it is transmitted, which would be the most effective preventive measure against lyme disease among a stable of racehorses?

Answers

The most effective preventive measure against Lyme disease among a stable of racehorses is Spraying down the stables regularly with an insecticide designed for ticks.

After removing the tick Abigail Bartholomew begins on antibiotics. Abigail worries that an open wound from a tick will lead to sepsis. The vascular system also called the circulatory system consists of blood vessels that carry blood and lymph throughout the body.

Arteries and veins carry blood throughout the body deliver oxygen and nutrients to the body's tissues and remove waste products from the tissues. Although many young animals can die the disease is usually not fatal in adult animals. However, it causes severe pain and suffering, especially in cattle. Animals can become permanently lame and recovering animals can be less productive.

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The US Memorial Holocaust Museum notes that 6 million Jews were killed during the Holocaust. From where do they MOST likely get this number? In the previous section, we saw a molecule made of three atoms to have a linear shape. In this section, we have observed a three atom molecule with a bent molecular shape. What is causing the difference in the two shapes?. In the diagram below, OQ = 25, PM = 9, and QN = 15. Find the length of OP.Round your answer to the nearest tenth if necessary.0Answer: OP |PMQNSubmit Answer how do I come up with the number let lines x and y be parallel, and let lines z and w be parallel. if angle a is the angle formed by lines y and z, and angle b is the angle formed by lines w and x, if the measure of angle a is 25 degrees, what is the measure of angle b? describe this picture but imagine yourself as one of the kids in the picture. Make use of all the 5 senses and everything you feel from this image. 200-250 words. Use first person prespective, develop a story around it as well. Paragraph style.if you solve this I'll mark brainliest which statement made by a nursing student indicates effective learning about which intervention is included in a plan of care for a patient with chronic kidney disease who is taking gluconate and calcium acetate? hesi the onset of increased the entertainment content in traditional news outlets as audiences for those outlets began to shrink. What mode of nutrition distinguishes animals from fungi, both of which are heterotrophs?. the number of women who successfully navigate the labyrinth is on the rise. group of answer choices true false A pet store sells a large dog kennel 98.50 the wholesale cost o the kennel is 63.55 what is the percent of markup round to the nearest percent. Daryl walks 10.5 miles in 3.5 hours. How many miles does he walk per hour? (Please Help!) For each quantity decide whether circumference or area would be needed to calculate it.A: The distance around a ferris wheelB:The amount of paint needed to paint a bullseyeC: The amount of whipped cream needed to go around the edge of a pieD The amount of material needed to make a drum head (the part of the drum you hit). you are an it administrator who is troubleshooting a computer from within windows command prompt. you are trying to execute the chkdsk command line tool, but it keeps failing. Use synthetic division to find the quotient and remainder when -x^4+2 x^3+12 x^2-16 is divided by x+2 by completing the parts below. Qualitative research often involves what's known as an iterative process for data collection and analysis. This terminology refers to the fact that, in qualitative research: Kyle needs 164 beads for a project. The beads are sold in packs of 2, 5, or 10. What is the least number of packs Kyle needs to buy. Explain An 80.0 g sample of a gas was heated from 25 C to 225 C. During this process, 346 J of work was done by the system and its internal energy increased by 6265 J. What is the specific heat of the gas? The time it takes an object to drop to the ground when in flight is __?__ as the time it takes to drop to the ground if you just drop it.A. less than B. more thanC. the same light is refracted through a diamond. if the angle of incidence is 30, and the angle of refraction is 12, what is the index of refraction? the most important step in making sure the proper amino acid is added to a growing polypeptide chain is: