low extracellular ph activates trpv1 through the binding of h ions to trpv1. describe the properties, including the location, of the site of trpv1 that allow h ions to bind. describe the specific location in the cell where atf3 protein is produced.

Answers

Answer 1

TRPV1 property TRPV1 protein is a ligand-gated ion channel that consists of several transmembrane domains embedded in the membrane, as well as a carboxy-terminus and an amino-terminus that are both present inside the cell ( cytosolic side ).

What is a binding site?

The region of an enzyme's surface (or receptor, or binding or transport protein) that contains the substrate or product (or other ligand).

The TRPV1 property TRPV1 protein is a ligand-gated ion channel composed of several transmembrane domains embedded in the membrane, as well as a carboxy-terminus and an amino-terminus found within the cell ( cytosolic side ).

In human cells, this causes division to halt and apoptosis to occur. ATF3 is a cAMP-dependent transcription factor that is encoded by the ATF3 gene located within the nucleus.

Thus, this is the the specific location in the cell where ATF3 protein is produced.

For more details regarding binding site, visit:

https://brainly.com/question/24302302

#SPJ1


Related Questions

1. What is the most astonishing forces on earth?

Answers

The strong nuclear force, additionally known as the robust nuclear interaction, is the most powerful of the 4 essential forces of nature. It's 6 thousand trillion trillion trillion (it truly is 39 zeroes after 6!) instances more potent than the pressure of gravity.

The nuclear pressure is a pressure that acts among the protons and neutrons of atoms. The nuclear pressure is the pressure that binds the protons and neutrons in a nucleus together. This pressure can exist among protons and protons, neutrons and protons or neutrons and neutrons. This pressure is what holds the nucleus together.

To learn more about nuclear force, click here:

https://brainly.com/question/19734564

#SPJ1

In the lac operon, lactose is a/an __________ and in trp operon tryptophan is a/an __________.

Answers

In the lac operon, lactose is an Inducible operon and in trp operon tryptophan is a Repressor operon.

For the transport and metabolism of lactose in E. coli and many other enteric bacteria, the lactose operon is a crucial operon. In E. coli and other bacteria, the lac operon is an operon or a collection of genes with a single promoter that codes for genes involved in lactose transport and metabolism. An inducible operon is the lac operon. Inducible operons are typically inactive and only activate in the presence of an inducer (lactose).

A repressor protein that binds to two tryptophan molecules activates the trp operon. The gene expression of the enzymes required to manufacture tryptophan is controlled by the tryptophan operon.

To know more about operon visit the link:

https://brainly.com/question/14331566?referrer=searchResults

#SPJ4

based on the data and observations obtained by your lab group and your working group, identify the optimal solvent to separate the food dye. purpose and explanation for why the chosen solvent was the bet

Answers

The vast majority of synthetic food colors are excellent candidates for CE (CAPILLARY ELECTROPHORESIS) separation. This is because they frequently include sulfonic acid or carboxylic acid functional groups, which at alkaline pH generate negatively charged colored ions, giving a unique chromophore for selective detection.

CAPILLARY ELECTROPHORESIS:

Synthetic food dyes are frequently used to improve the look of meals and have several advantages over natural colorings, including being brighter, more stable, less expensive, and accessible in a wider range of hues. However, the revelation of some food colors' harmful effects has resulted in a substantial decrease in the range of synthetic colors that are approved food additives in certain regions of the world. The vast majority of synthetic food colors are excellent candidates for CE separation. This is because they frequently include sulfonic acid or carboxylic acid functional groups, which at alkaline pH generate negatively charged colored ions, giving a unique chromophore for selective detection. However, their examination is complicated by the fact that many food colors contain a combination of components rather than a single chemical species. Although documented techniques are less common, CE has been used to analyze natural colors such as caramels, which are classified into four unique groups based on the reactants utilized during sugar caramelization. In fact, CE analysis can be used to identify and quantify the type of caramel in a sample.

To know more about CAPILLARY ELECTROPHORESIS visit the link:

https://brainly.com/question/14285961

#SPJ4

What kind of change results from the breakdown of starch into glucose by saliva?.

Answers

Chemical change results from the breakdown of starch into glucose by saliva.

Chemical changes occur when one substance combines with another to form a new substance, which is known as chemical synthesis, or when two or more different substances combine to form a new substance, which is known as chemical decomposition. These processes are known as chemical reactions, and they are generally irreversible except through further chemical reactions. Chemical changes include burning, cooking, rusting, and rotting.

Important chemical reactions in biological systems include: Water and CO2 combine to form glucose and oxygen. The process of converting glucose into carbon dioxide and water. Oxyhaemoglobin is formed by the reaction of haemoglobin and oxygen.

Amylases break down starch into smaller molecules, producing maltose, which is then cleaved into two glucose molecules by maltase.

To learn more about chemical changes, here

https://brainly.com/question/23693316

#SPJ4

The external brain structure found between the pre-central gyrus and the post-central gyrus is the.

Answers

The primary motor cortex would be located in the cerebral hemisphere, which would be rostral to the central sulcus. The primary somatosensory cortex is located in the postcentral gyrus, which would be caudal towards the central sulcus.

On the medial portion of the hemisphere, the central sulcus frequently extends for a little distance. The paracentral lobule is a physical structure formed by the medial expansions of the precentral as well as postcentral gyri, which are located anteriorly and posteriorly to it.

The cerebral hemispheres, the cerebellum, as well as the caudal or medullary region of the brainstem were the three main components that can be seen in most images of the human brain.

To know more about postcentral gyrus

https://brainly.com/question/13062757

#SPJ4

Earth experienced a ‘global warming’ event 55 million years ago. What is the likely cause of the final, dramatic pulse of warming during this time?.

Answers

The greenhouse gases were the major cause of the ‘global warming’ event 55 million years ago.

Over 55 million years ago, as a result of human lives being developed, major greenhouse gases such as carbon dioxide and carbon monoxide began to expand on the Earth. This caused a dramatic change in the Earth's conditions causing global warming.

Greenhouse gases are gases that are harmful to the earth and damage it. The greenhouse gases also increase the temperature of the earth hence making it unsuitable for living conditions. One of the leading causes of global warming is the emission of greenhouse gases.

To learn more about global warming, click here:

https://brainly.com/question/3553382

#SPJ4

the function of sexual reproduction is to increase variation within populations. meiosis is the molecular mechanism that leads to sexual reproduction. as such, some of the features that make it unique lead to genetic diversity. which of these unique features are responsible for increasing genetic diversity?

Answers

The two distinctive processes that increase genetic variety are homologous recombination and reduced division.

How does genetics work?

The study of genes and heredity, or how particular characteristics or traits are passed from mother to baby as a result of changes in DNA sequence, is known as genetics. A gene is a section of DNA that has the instructions needed to create one or more molecules necessary support bodily function.

What leads to genetic illness?

Mutations in one gene (monogenic disorder), multiple genetic material (multifactorial inheritance disorder), a combination of gene mutations in genes, or damage to genes changes in the number and structure of chromosome 21, the structures that comprise a person's DNA) can all result in genetic disorders.

To know more about genetic  visit:

https://brainly.com/question/8868332

#SPJ4

DNA Sequence CCT CTT TAC ACA CGG AGG GTA CGC TAT TCT ATG ATT ACA CGG TTG CGA TCC ATA ATC
Create an mRNA sequence from the above DNA sequence

Answers

An mRNA sequence from the given DNA sequence is GGA GAA AUG UGU GCC UCC CAU GCG AUA AGA UAC UAA UGU GCC AAC GCU AGG UAU UAG.

DNA consists of two types of strands. One is the coding strand and the other is the template strand. The coding strand is not transcribed to mRNA and is directed in a 3' to 5' direction. The template strand is transcribed to mRNA and is directed in a 5' to 3' direction.

Here, consider the given strand as a template strand. Our mRNA strand will be complementary to this strand. Instead of thymine, mRNA will have uracil.

According to genetic code, the nucleotides in the sequence are arranged into three codes called codons in mRNA.

Therefore,

DNA sequence: CCT CTT TAC ACA CGG AGG GTA CGC TAT TCT ATG ATT ACA CGG TTG CGA TCC ATA ATC

mRNA sequence: GGA GAA AUG UGU GCC UCC CAU GCG AUA AGA UAC UAA UGU GCC AAC GCU AGG UAU UAG

To know more about codon:

https://brainly.com/question/22991780

A F1 plant that is homozygous for tallness is crossed with a heterozygous F1 plant. What ratio of tall to short plants (tall:short) represents the possible offspring?
A.
1:3

B.
4:0

C.
1:1

D.
3:1

Answers

Answer:it is c

Explanation:it just is

Explain the relationship between a P and S wave that makes
it possible to determine the location of an earthquake focus.
How is the focus location determined?

Answers

Answer:Use the time difference between the arrival of the P and S waves to estimate the distance from the earthquake to the station.

Explanation:

Which of the following observations best supports the claim that mitochondria evolved from once-free-living prokaryotic cells by the process of endocytosis?
A. Mitochondria produce ATP.
B. Mitochondria contain proteins.
C. Mitochondria exchange substances with the cytosol.
D. Mitochondria are surrounded by a double membrane.

Answers

Option D. Mitochondria are surrounded by a double membrane, observations best support the claim that mitochondria evolved from once-free-living prokaryotic cells by the process of endocytosis.

Prokaryotes are unmarried-celled organisms belonging to the domains bacteria and Archaea. Prokaryotic cells are much smaller than eukaryotic cells, have no nucleus, and lack organelles. All prokaryotic cells are encased by way of a cell wall. Many even have a pill or slime layer made of polysaccharides.

A prokaryote is a single-celled organism that lacks a nucleus and different membrane-sure organelles. The word prokaryote comes from the Greek within the -empire system bobbing up from the paintings of Chatton, prokaryotes had been categorized in the empire Prokaryote.

Learn more about prokaryotic cells here:-https://brainly.com/question/5716507

#SPJ1

the two most basic steps of dna replication are

Answers

Explanation:

The initiation of DNA replication occurs in two steps. First, a so-called initiator protein unwinds a short stretch of the DNA double helix. Then, a protein known as helicase attaches to and breaks apart the hydrogen bonds between the bases on the DNA strands, thereby pulling apart the two strands.

An individual with the genotype aabb makes gametes. An individual with the genotype aabb also makes gametes. How many gamete types do they have in common with respect to these two genes?.

Answers

An individual with the genotype AaBB and an individual with the genotype AaBb both make gametes. 2 types of gametes they have in common with respect to these two genes.

A dihybrid cross is a mating experiment between two organisms that are genetically identical in two ways. A heterozygous hybrid organism is one that carries two different alleles at a specific genetic position, or locus. Mendel used a dihybrid cross to combine two opposing traits, such as the color and shape of seeds, for crossing. He crossed the wrinkled-green seed with the round-yellow seed. In the F1 generation, he only got round-yellow seeds.

The term F1 generation refers to the offspring produced by a cross between two different types.

To learn more about Dihybrid cross, here

https://brainly.com/question/12541734

#SPJ4

Which of the following host defenses is considered the most effective in combating S. aureus infection?

Answers

Phagocytic response is considered the most effective host defenses in combating S. aureus infection.

What is phagocytic response?

Phagocytosis is a type of cell response that plays a key role in the course of an immune response as well as in the remodeling of tissues and the healing of wounds. Professional phagocytes are specialized cells that can carry out this task quite effectively.

What is S. aureus infection?

It has long been known that S. aureus is one of the most significant germs that harm humans. It is the main contributor to skin and soft tissue infections such cellulitis, furuncles, and abscesses (boils). Boils are the most typical staph infection form. This is a pus-filled pocket that forms in an oil gland or hair follicle. Typically, the skin around the infected area turns red and swells. To treat staph infections, doctors frequently administer cefazolin, nafcillin, oxacillin, vancomycin, daptomycin, and linezolid. Vancomycin may be necessary for staph infections that are severe. This is due to the fact that a large number of staph bacterium strains have developed resistance to other common antibiotics.

Thus from above conclusion we can say that phagocytic response is considered the most effective host defenses in combating S. aureus infection.

Learn more about the phagocytic response here:

https://brainly.com/question/28232491

#SPJ4

Can someone help me with this?

Decomposition


Displacement


Synthesis


Elimination

Answers

Answer:

displacement reaction

Explanation:

because in the chemical equation CuSO4+Mg, Cu and Mg switch places so the equation is now MgSO4+Cu. This is an example of a single replacement because only one pair of elements switch with each other

1.
2.
3.
going across
i’m confused please help :(

Answers

Leukocytes, thrombocytes, and erythrocytes are seen in the provided image. Thrombocytes, often known as platelets, help to clot blood. Red blood cells, or erythrocytes, are crucial for carrying oxygen throughout the body. Leukocytes, or white blood cells, also have a significant impact on the immune system.

Blood cells come in three main categories: erythrocytes (red blood cells), leukocytes (white blood cells), and thrombocytes (platelets). Leukocytes are the primary cellular immune system components that contribute to the body's defence against invading pathogens, while Thrombocytes are involved in the blood clotting process that prevents excessive bleeding. Erythrocytes fulfil the oxygen needs of all cells during the transportation process. Erythrocytes, Leukocytes, and Thrombocytes differ mostly in this way.

Leukocytes, thrombocytes, and erythrocytes can thus be identified as the first, second, and third cell types in the provided image, respectively.

LEARN MORE ABOUT BLOOD CELLS HERE:

https://brainly.com/question/17890844

#SPJ1

the cribriform plate supports the - the terminus of the - and is perforated by numerous for the passage of the branches of the . b. olfactory bulbs of cn 01 i. are olfactory bulbs seated superior or inferior to the cribriform plate? ii. zoom in to see the projections on the inferior surface of the bulbs. (tip: you may need to hide the ethmoid bone to see them well.) iii. within each bulb, axons of olfactory form synapses with dendrites and cell bodies of other . iv. if you are attempting to view the olfactory bulbs on a preserved specimen, they may be missing or incomplete. why do you think this is? c. olfactory tracts of cn 01 - olfactory tracts are formed by axons/dendrites (circle one). d. lateral stria of cn 01 - follow this tract to see which region of the brain it terminates in: e. medial stria of cn 01 - follow this tract to see which region of the brain it terminates in: f. signals transmitted along the olfactory tracts terminate in the area in the lobe of the cerebral cortex. 3. based on your learning, how do you think damage to this nerve would present clinically? 4. what

Answers

The cribriform plate, that is pierced by several passageways of a (b) olfactory bulbs, serves as support for it (ii). View a protrusion on the underside of the bulbs by zooming in.

What is cribriform plate ?

At the skull base, on the ethmoid bone, is the cribriform plate. The most inferior part of the skull is referred to as the the skull's base. It is made up of pieces of the temporal bone, occipital bone, sphenoid bone, ethmoid bone, and frontal bone. The cribriform plate, also known as the horizontally lamina and lamina cribrosa in mammals, is a component of the ethmoid bone. It is taken up by the frontal bone's ethmoidal notch and ceilings of the nasal cavities. It support the olfactory bulb and has olfactory foramina that allow the olfactory nerve to pass through and reach the roof of a nasal cavity, where they transmit smell to the brain. While a foramina just at lateral portion of the groove transmit nerves to a superior nasal concha, those who are at the middle portion of the groove allow nerves to travel to the upper section of the nasal septum.

Where is the cribriform plate and what function is it associated with?

The anterior cavity as well as the nasal cavity are separated by a structure like a sieve called the cribriform plate, which is also often referred to as the laminar cribrosa of such ethmoid bone. The olfactory bulb, that is located in the olfactory fossa, is supported by this ethmoid bone component. The olfactory nerves which carry the smell sense are transmitted by this bones, the cribriform plate.

To know more about Cribriform Plate visit:

https://brainly.com/question/24357262

#SPJ4

why are chromosomes cut into fragments for sequencing?

Answers

Prior to DNA sequencing, chromosomes are broken up because only about 500 bases can be reliably called by DNA sequencing reactions.

Only roughly 500 DNA bases can be properly determined by a DNA sequencing process. The DNA of a cell is broken into relatively big chunks using a technique called map-based sequencing. After a certain length, it becomes challenging to determine the precise base or nucleotide. This is due to the fact that the length of the DNA strand and base quality are inversely related. Enzymatic DNA fragmentation techniques use enzymes to break intact DNA and long sequences into perfect fragments for sequencing, producing clearer findings than subdividing longer DNA sequences into smaller bits before sequencing. Transposase, restriction, and nicking enzymes are some of the enzyme-based techniques for DNA fragmentation for sequencing.

Learn more about to fragmentation visit here:

https://brainly.com/question/22052154

#SPJ4

cyamids suddenly suffer a widespread population drop. what effect will this have on right whales?

Answers

Cyamids suddenly suffer a widespread population drop Yosemite.  

These small crustaceans also referred to as whale lice or cyamids, feed on the whales' pores and skin. Incapable of swimming or surviving inside the open water, they tour from one whale to another by direct contact.\

Whale Cyamids are outside parasites that stay at the frame floor of such marine mammals as whales, dolphins, and porpoises. They take shelter in pores and skin lesions, genital folds, nostrils, eyes, and different external orifices, feeding on host tissue or fluid secretions.

While hitching a trip, these crustaceans munch on algae and whale pores and skin. although that might sound like an undesirable scenario for a whale, a few researchers believe there is no proof that whale lice are unfavorable to whales, and accordingly have extra commensal dating with their hosts, like barnacles.

Learn more about Cyamids  here:

https://brainly.com/question/16970511

#SPJ4

what do we depend on the environment for?​

Answers

I think it is oxygen
Food
stable climate
shelter
water

you have discovered a new enzyme, but when you isolate it for further study, it does not work properly. what might have been lost?

Answers

You have discovered a new enzyme, but when you isolate it for further study, it does not work properly. The coenzyme have been lost.

What is a coenzyme?

A coenzyme is classified as an organic molecule that combines to the active locations of an enzyme and helps to catalyze the chemical reaction rate.

More specifically, coenzymes function as electron carriers or for transferring hydrogen during chemical reactions.

For example, nicotineamideadenine dinucleotide, and flavin adenine dinucleotide are coenzymes.

Simply put, coenzymes are non-proteins that are attached to the enzymes as intermediate carriers and act as catalysts to speed up the rate of reaction.

If you need to learn more about enzymes click here:

https://brainly.com/question/14577353

#SPJ4

Dna replication begins at a site called the origin of replication, forming a bubble, which is followed by a __________, where parental strands are unwound by __________.

Answers

DNA replication begins at a site called the origin of replication, forming a bubble, which is followed by a replication fork, where parental strands are unwound by helicases.

Each time a cell divides, the two resulting daughter cells must contain the same genetic information, or DNA, as the parent cell. To accomplish this, each strand of existing DNA acts as a template for replication.

Replication occurs in three major steps: the opening of the double helix and the separation of the DNA strands, the priming of the template strand, and the assembly of the new DNA segment.

DNA helicases catalyze the disruption of the hydrogen bonds that hold the two strands of double-stranded DNA together. The replication fork is a multiprotein complex with helicase and DNA synthesis activities.

Learn more about DNA replication here:

https://brainly.com/question/112

#SPJ4

How do bonds form between atoms?


What Is a Chemical Bond?

A chemical bond is a force of attraction between atoms or ions. Bonds form when atoms share or transfer valence electrons. Valence electrons are the electrons in the outer energy level of an atom that may be involved in chemical interactions. Valence electrons- electrons furthest from the nucleus, are the basis of all chemical bonds.



Question: How do bonds form between atoms?


Sharing or transferring neutrons

Sharing or transferring electrons

Sharing or transferring protons

Answers

Bonds form by sharing or transferring electrons.

It’s in the text if you just read it!


5. Which organism is gaining the most energy in this pyramid?
6. Which organism is gaining the least energy in this pyramid?
7. How much energy is available to the snake?

Answers

In the given pyramid of energy, the organism gaining most energy is plants, the organism gaining least energy is eagle and according to the 10% rule of energy, the snake will have only 10% of the total energy of rat.

Pyramid of energy is the diagrammatic representation of the transfer of energy at each trophic level in a food chain. The pyramid of energy is always upright. This is because the organism at the lowermost level has the maximum energy and the organism at the topmost level will have the least.

10% rule of energy transfer states that only 10 % of the total energy is transferred from one organism to another during a food chain. The organism at first trophic level, i.e. plants will also be able to absorb only 10% of the total sun's energy. Similarly rat will have only 10 % of energy available to it because the rest gets lost in respiration, digestion, in the form of heat, etc.

To know more about pyramid of energy, here

brainly.com/question/2515928

#SPJ1

What causes the differentiation of blood stem cells into new cells with different shapes and functions?.

Answers

Signaling mechanisms cause the differentiation of blood stem cells into new cells with different shapes and functions.

A cell just needs to modify the genes (and consequently the proteins) that will be expressed in order for it to differentiate into its specific form and function. Transcription factors are the main process by which genes are turned "on" or "off." Cellular interactions with the surrounding matrix, growth and differentiation factors, and other environmental factors that temporally and spatially activate or inhibit cellular signaling pathways and the transcription of particular genes drive this complex process.

Pluripotent cells called embryonic stem cells (ESCs) can differentiate as a result of signaling pathways. Most growth factors, cytokines, and epigenetic processes including DNA methylation and chromatin remodeling tightly regulate them.

To know more about stem cells visit the link:

https://brainly.com/question/25584485?referrer=searchResults

#SPJ4

The region of the brain that encloses the third ventricle and immediately rostral to the brainstem, and includes the thalamus, epithalamus, and hypothalamus is the.

Answers

The region of the brain that encloses the third ventricle and immediately rostral to the brainstem, diencephalon  .

Brainstem is the bottom, stalklike portion of your brain. It connects your mind on your spinal cord. Your brainstem sends messages to the rest of your body to adjust stability, respiration, heart price and greater. surprising accidents, and mind or coronary heart situations may additionally have an effect on how your brainstem w

The brainstem (mind stem) is the distal a part of the mind that is made up of the midbrain, pons, and medulla oblongata. every of the 3 components has its very own specific structure and feature. collectively, they assist to adjust respiratory, coronary heart price, blood stress, and several other critical features.

A brain stem damage can purpose dizziness or lack of motor feature, with extra severe instances resulting in paralysis, coma, or dying. remedy can be extraordinarily pricey, and plenty of sufferers are not able to paintings whilst handling a mind stem harm.

Learn more about brainstem  here:

https://brainly.com/question/1360688

#SPJ4

global sea level during the mid-cretaceous was over 200 m higher than today, reducing the total land size and increasing the rate of extinction of land-animal species. what caused this increase in the sea level?

Answers

The cause of this increase in the sea level is Increased rates of seafloor spreading

What happens to cretaceous when sea level increase ?

The most well-studied mass extinction, which occurred 66 million years ago at the beginning of the Paleogene and the Cretaceous epochs, wiped out the nonavian dinosaurs and cleared the way for the rapid diversification and evolution of mammals and birds.

The "Big Five" extinctions are most well-known for the end-Cretaceous event, which saw the extinction of all dinosaurs except for birds. Additionally, it gave mammals opportunities. Dinosaurs ruled all land habitats during the Mesozoic Era.

Contrary to earlier extinction events that were brought on by natural phenomena, the sixth mass extinction is being caused by human activity, particularly (though not exclusively) the unsustainable use of land, water, and energy, as well as climate change.

Learn more about Cretaceous here:

https://brainly.com/question/505969

#SPJ4

The prime factorization is 3 2 ・5 2 . What number does this describe?

Answers

Answer:

scuse me but this is math

Which statement is FALSE?
-When the open promoter complex is formed, sigma 1.1 is displaced from DNA channel and replaced by DNA.
-As bent DNA template is in the RNAP active site, it presses on the sigma 3.2 domain, thus releasing it from the holoenzyme.
-As DNA enters the channel in the RNAP active site, it begins to bend 90 degrees and melt as the strands open up close to start of transcription.
-In the free holoenzyme of bacterial RNAP, the N-terminal domain of sigma-70 blocks DNA channel by mimicking interaction with DNA.

Answers

As bent DNA template is in the RNAP active site, it presses on the sigma 3.2 domain, thus releasing it from the holoenzyme is wrong among other statements.

What is RNAP active site?

During transcript elongation, RNA polymerase II may become halted, in which case the ternary complex is unaffected but additional RNA synthesis is prevented. The emerging transcript needs to be split off at the 3' end in order to release arrest.

Transcription continues from the freshly exposed 3' end as 7–17 nt RNAs are freed. The transcript cleavage reaction is substantially stimulated by factor SII, which boosts elongation efficiency. Here, we demonstrate how the addition of pyrophosphate can likewise result in the release of an arrest.

The same set of cleavage products are formed as by factor SII, but those produced by pyrophosphate have termini that are 5'-triphosphate.

To know more about RNA polymerase, visit:

https://brainly.com/question/24268781

#SPJ4

After transformation of e. Coli with pglo, why are the cells grown in lb broth for 20 minutes before plating on amp plates?.

Answers

After transformation of E.Coli with pGLO, It takes 20 minutes to transcribe and translate the Green Fluorescent Protein.

Before RNA polymerase can bind to the Arabinose promoter, it must wait at least 20 minutes. There is a 20-minute phenotypic lag before the b-lactamase gene for Amp resistance can be expressed. The pGLO plasmid contains a gene (bla or ampR) encoding a protein known as -lactamase, which hydrolyzes antibiotics with a-lactam ring and renders host cells resistant to compounds such as ampicillin. The pGLO Lab Kits make use of Bio-pGLO Rad's plasmid, which encodes a green fluorescent protein (GFP), to give instructors a hands-on introduction to transformation, cloning, protein chromatography, and electrophoresis techniques. The pGLO plasmid contains the following genes: GFP, bla, Ori (origin of replication), araC, pBAD promoter, and a region with multiple cloning sites.

To learn more about pGLO, here

https://brainly.com/question/14730325

#SPJ4

Other Questions
What is the difference between Cell Differentiation and Mitosis? after a buyer expresses a price objection, it is inappropriate for the salesperson to ask the buyer probing questions. a.true b.false Chickarees, or Douglas squirrels, live in northwest pine, spruce, and fir forests. To feed, they gnaw through the stems of green cones. The cones fall to the ground, and the squirrels climb down after them. They dig seeds from the cones, or they hide the cones away to save.The chickaree's loud, chattering call is similar to that of the eastern red squirrel. Its coloring is reddish-brown on the underside, with upper parts rusty in winter, and in summer olive brown with a black line on the sides.Offspring are born in June, usually five to a litter. By fall, the young can care for themselves.QuestionAccording to the passage, how does a chickaree get food?Answer options with 4 options1.It digs seeds out of the ground and carries them up into its tree to eat.2.It chews cones off the trees and follows them to the ground to pick out the seeds.3.It waits for the cones to fall from a tree, then gathers them and stores them for winter.4.It steals cones from other animals who harvest them and digs the seeds out of the cones. For each of the following situations, discuss the reasons you may or may not need an attorney.: 1. You hit another car in a parking lot. Your insurance agent indicates that the company will pay for bodily injury and property damage.2. You borrow a friend's car without his knowledge, and he reports it to the police as stolen. 3. You buy a new laptop for $800. One month later, the laptop stops working. You return to the store, and the salesperson tells you he is sorry but these laptops have only a two-week guarantee.4. You decide to trade in your old car and buy a new one.5. Two friends are caught robbing the cashier at a local store, and they name you as someone who helped plan the robbery. 6. You are turned down when you apply for a job. You think you were rejected because you are deaf. 7. You do not want your family to inherit the $10,000 you have saved. After being told you will die within a year, you want the money to be used for cancer research. 8. You and your spouse can no longer get along. You want a divorce. 9. You earn $5,000 working in a restaurant during the year. You want to file your federal income tax return.I need a answer for each one please. what is the name of the hormone producing cells in the middle of this endocrine organ, indicated by the arrow? 2. How were the British soldiers involved in the incident? What about the colonist?what is..British Point of View:Americans Point of View:Topic: Propaganda in American History The Boston Massacre How do I find the right degree angle of a triangle? The length of a rectangle is 5 m longer than its width.If the perimeter of the rectangle is 70 m, find its length and width.length: mwidth: m The team uses its ________ to determine how many requirements it can commit to accomplishing in the next scrum period. A scale drawing of a rectangular bedroom is shown below. Using a scale of 1cm=4 feet, enter the actual area of the bedroom in square feet. Veterans day was originally celebrated one year after the completion of which war?. Green plants use light from the Sun to drive photosynthesis. Photosynthesis is a chemical reaction in which water (HO) and carbon dioxide (CO)chemically react to form the simple sugar glucose (C6H206) and oxygen gas (O).What mass of water is consumed by the reaction of 8.86 g of carbon dioxide?Be sure your answer has the correct number of significant digits. if evan does a job in 11 hours and with the help of himari they can do it together in 6 hours, how long would it take himari to do it alone? calculate the response of an undamped system with stiffness k and mass m to the excitation given in fig. 7, assuming the system is initially at rest. you want to create a portfolio equally as risky as the market, and you have $500,000 to invest. information about the possible investments is given below: asset investment beta stock a $ 145,000 .90 stock b $ 135,000 1.35 stock c 1.50 risk-free asset a mother arrives at the hospital emergency department with her child, in whom a diagnosis of epiglottitis is documented. which prescription, if written by the primary health care provider, should the nurse question? which part of the flower is labeled with an x if sales in 2003 were $300,000 and they grew to $500,000 by 2007 (a four-year period), the actual annual compound growth rate was: According to the map of electoral college votes per state, which state has the LEASTelectoral college votes? Which statement best completes the timeline of events in the RevolutionaryWar?The Patriotsdefend aweaponsstorehouse.The Battles ofLexington andConcordThe Patriots wintheir first battleand morale israised.The Battle ofTrentonThe British areforced toabandon thenorthern strategy.The Battle ofSaratoga??A French fleetblocks Britishreinforcements.The Battleof YorktownA. The Battle of Bunker Hill: The British launched an offensive attackat Breed's Hill.OB. The Battle of Quebec: All Patriot forces are forced to pull out ofCanada.OC. Winter at Valley Forge: Washington's soldiers endure a harshwinter.OD. The Battle of Kings Mountain: The Patriots first use the "hit-and-run tactic.