If you forgot to include primers in your pcr reaction do you think that your target dna would be amplified?.

Answers

Answer 1

NO if forgot to include primers in pcr (polymerase chain reaction) reaction it wont work

Polymerase chain reaction is based on harnessing the ability of a DNA polymerase to synthesize a new DNA strand complementary to a provided template strand. DNA polymerases can only add nucleotides to an already existing 3'-OH group, so a primer that can add the first nucleotide is required. The main components of a PCR reaction are Taq polymerase, primers, template DNA, and nucleotides (DNA building blocks). The components are assembled in a tube along with the necessary cofactors for the enzyme and undergo repeated heating and cooling cycles that allow DNA synthesis.

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Related Questions

what is an index fossil? group of answer choices a marker used to determine the age of a geological layer the ideal specimen of that species that all later descriptions must refer to the type specimen of a species a fossil found in a particular site

Answers

An index is a fossil use to categories a stratigraphic layer.

What is index fossil ?

Geological periods are identified using index fossils. The term "commonly found, widely distributed fossils that are limited in time span" can be used to describe these fossils. One can estimate the age of a layer if they discover an index fossil in it. The geological eras are identified by using index fossils.

Index fossils are the remains of organisms that lived briefly and widely, leaving behind a large number of fossils. Scientists can match rock layers using index fossils. The age of two rock layers that contain the same index fossil but are located in different locations is similar.

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a(n) cell contains two sets of chromosomes, one set of chromosomes inherited from each parent. multiple choice question.

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A diploid cell contains two sets of chromosomes, one set of chromosomes inherited from each parent.

A diploid cell contains two full sets of chromosomes. With 23 chromosomal pairs totaling 46 chromosomes, diploid human cells predominate. 22 pairs of autosomes and a pair of sex chromosomes make up this.

Chromosomes are arranged in homologous pairs in diploid cells. The term "diploid chromosomal number" refers to how many chromosomes are present in a cell as a whole (2n). In a diploid human cell, there are 46 chromosomes altogether since in this case, 2n = 46.

The DNA from both of the organism's parents makes up the homologous chromosomal pairs; the mother and father each contribute one chromosome to each pair.

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Some researchers suggest that ______ molecules were the first information molecules formed on early earth.

Answers

Answer: RNA

Explanation:

The RNA world hypothesis suggests that life on Earth began with a simple RNA molecule that could copy itself.

During photosynthesis, the energy used to pump protons comes from ___________, whereas in cellular respiration it comes from _____________.

Answers

During photosynthesis, the energy used to pump protons comes from ______light_____, whereas in cellular respiration it comes from ______NADH/FADH₂_______.

What are the steps in photosynthesis?The first step in photosynthesis is the absorption of light by chlorophyll bound to chloroplast thylakoid proteins. The absorbed light energy is used to remove electrons from electron donors such as water to form oxygen.The electrons are then transferred to the primary electron acceptor, quinine (Q.). Electrons are further transferred from the primary electron acceptor to the final electron acceptor (usually NADP⁺).Proton transfer from the thylakoid lumen to the stroma via the F₀F₁ complex generates ATP from ADP and Pi.The NADP and ATP produced in steps 2 and 3 provide the energy, and the electrons power the process of reducing the carbon to a six-carbon sugar molecule.

The first three steps of photosynthesis, are directly dependent on light energy and are thus, called light reactions, while the reactions in the last step are independent of light and thus are termed dark reactions.

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The trend for women to delay childbirth until their late 20s or early 30s has ___________ while the peak years for female fertility are ____________.

Answers

While the peak years for female fertility are declining, the trend for women to put off having children until their late 20s or early 30s has a high fertility rate.

Is childbirth more difficult in your 30s?

After you reach your mid-30s, pregnancy is something to think about more. Women who are older have a higher risk of miscarriage or stillbirth. They are more likely to experience preeclampsia, gestational diabetes, and to give birth to a very little child.

When do women start having children?

The prime reproductive years for women are from their late teens to late 20s. Age 30 marks the onset of a reduction in fertility (the capacity to become pregnant). Once you're in your mid-30s, this drop accelerates.

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Which of the following best describes the genetic material a person receives from each parent?

Answers

I'm pretty sure an x or y chromosome

perspective that our genes and environmental influences work together to determine our characteristics

Answers

Environmental factors often influence traits independently of genes. Either of these effects can change the proteins that are made from a gene, which in turn affects traits.

How do environment and gene determine our personality?

Some genes increase the characteristic and others work to decrease the same characteristic-complex relationship among the various genes, as well as a variety of random factors, produce the final outcome.

Because of their genetic makeup, individuals differ in their responsiveness to the qualities of the environment. There are unique, genetically influenced reactions to particular experiences to which we are exposed.

The presence of drugs and chemicals in an organism's environment can also influence gene expression in the organism. The environment can affect morphological and physiological development whereas genes influence morphology and physiology, creating a framework within which the environment acts to shape the behavior of an individual.

Hence in this way, genes and environmental influences work together to determine our characteristics.

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Why are soft-bodied organisms less likely to leave a fossil trace than hard-bodied organisms?.

Answers

The soft body parts of the organism are more likely to deteriorate when buried inside the earth and hence they do not leave any fossil trace than the hard-bodied organisms.

Fossils are the remains, traces or impressions of the animals and plants that dies millions of years ago and were buried deep down in the Earth. The fossils provide information about the organisms of those times and also help in establishing the ancestral history of current organisms and their evolution.

Evolution is the change in species that occurs in long periods of time for generations over generations. The term evolution is a population concept because evolution can never be seen in one individual but is visible in a population.

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Protists include?

a. yeasts and molds.
b. algae and protozoa.
c. helminths.
d. all of these choices are correct.

Answers

Protists consist of algae and protozoa. accurate choice is b. algae and protozoa. Algae are plant-like organisms that may be both unicellular or multicellular, and derive electricity through photosynthesis.

Protozoa are unicellular organisms with complicated mobileular structures; maximum are motile. Microscopic fungi consist of molds and yeasts. Helminths are multicellular parasitic worms. In the protist kingdom, there are important groups: protozoa (which might be normally heterotrophic) and algae (which might be normally autotrophic).

The essential distinction among protozoa and algae is that protozoa are heterotrophic, at the same time as algae are autotrophic. This manner that protozoa ought to locate and ingest their meals from their environment, at the same time as algae are photosynthetic and are capable of make their personal meals.

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A student is developing a model of sex cell formation.
X
X
XX
XX
X
t
DI
00
00
Which of the following conclusions can be made from the student's modol of why the dna of the daughter cells is different from the dna of the parent cell?

Answers

Answer:

Can you please include the answer choices....

Explanation:

Thanks. I'll answer it tho.

Why is photosynthesis and cellular respiration considered a cycle?

Answers

Answer:

Cellular respiration and photosynthesis are considered a cycle because the product (end material) of one reaction serves as the starting material for the other reaction

Explanation:

The starting material for cellular respiration is sugar and water and the products are carbon dioxide and water. Water and carbon dioxide are starting material for photosynthesis.

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2 liters of 2% sucrose solution from 10% concentrated stock solution

Answers

In order to get 2 liters of 2% sucrose solution from 10% concentrated solution, 0.4 liters of the stock would be taken and diluted with 1.6 liters of water.

Dilution

The problem here is that of a simple dilution. The dilution principle affirms that the number of moles before and after dilution must be constant.

[tex]m_1v_1[/tex] = [tex]m_2v_2[/tex]

where:

[tex]m_1[/tex] = initial concentration or concentration of the stock solution.

[tex]m_2[/tex] = final concentration or concentration of the diluted solution.

[tex]v_1[/tex] = initial volume or volume of the stock solution to be diluted.

[tex]v_2[/tex] = final volume or volume of the diluted solution

In this case:

[tex]m_1[/tex] = 10%

[tex]m_2[/tex] = 2%

[tex]v_2[/tex] = 2 liters

[tex]v_1[/tex] = [tex]m_2v_2/m_1[/tex]

    = 2 x 2/10

     = 0.4 liters

Thus, 0.4 liters of the 10% stock solution would be taken and diluted up to the 2 liters mark (by adding 1.6 liters of water) to make a 2% solution.

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Proper segregation of plasmids into daughter cells may incorporate which of the following strategies? o Random partitioning due to high copy number
o Polymerization of a filament that binds to, and physically separates plasmids
o Post-segregational killing via toxin / antitoxin system

Answers

Random partitioning due to high copy number, Polymerization of a filament that binds to, and physically separates plasmids and Post-segregational killing via toxin/antitoxin system are the ways incorporated to Properly segregate the plasmids into daughter cells.

Plasmids not only replicate, copying across to a daughter cell when a bacterium divides, but they can also pass between unrelated bacteria via mechanisms such as cell-to-cell contact or a 'bridging connection' in a process known as 'conjugation,' which has been described as the bacterial equivalent of sex.

Plasmid purification consists of three basic steps: bacterial culture growth, bacterial harvesting and lysis, and plasmid DNA purification. Low-copy number plasmids require a segregation mechanism to ensure that one-half of the plasmid copies are distributed to each daughter cell during cell division. This can be accomplished directly by partitioning plasmid copies via a mechanism similar to eukaryotic mitosis.

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What is the process in which an mrna molecule is used to synthesize a specific polypeptide on a ribosome?.

Answers

The process in which an m-RNA molecule is used to synthesize a specific polypeptide on a ribosome is called translation.

m-RNA stands for messenger RNA. It is the main coding enzyme that encodes the information from the DNA sequence to be translated to the protein. It is transcribed from the DNA with the help of enzyme RNA Polymerase II in eukaryotic cells.

Ribosome is a nucleoprotein. It is the main machinery for the synthesis of protein. The ribosome is composed of two subunits: a small subunit and  a large subunit. There are two types of ribosomes: 70 S type and 80 S type.

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Drag each tile to the correct location.
Sort the statements based on whether they describe DNA replication in eukaryotes or prokaryotes.
Replication takes place in
the nucleus.
Replication happens at just
one point on the chromosome.
There is only one origin of
replication.
Replication takes place
in the cytoplasm.
Prokaryotes
G
There are multiple origins
of replication.
Replication occurs at multiple
points along the chromosome.
Eukaryotes

Answers

The nucleus is where replication occurs in eukaryotes. Replication has several different origins. Along the chromosome, replication takes place several times. Prokaryotes replicate within their cytoplasm. Only one location on a chromosome experiences replication. There is just one origin for replication.

The process of DNA replication, which is managed by the enzyme DNA polymerase, involves the genetic material of a cell—in this case, the DNA—making a perfect copy of itself. In comparison to bacteria, which replicate at a pace of about 500 nucleotides per second, mammals replicate at a rate of about 50 nucleotides per second.

Prokaryotic DNA replication happens through a single origin of replication, whereas eukaryotic DNA replication occurs through numerous replication origins. This is the primary distinction between prokaryotic and eukaryotic DNA replication.

Thus, we can conclude that the location, complexity, and size of the cell have a role in the differences between DNA replication in prokaryotic and eukaryotic cells.

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HELP PLEASE!!! Match the correct term with the corresponding definition or description.

AND ANSWER ALL!!!!

Answers

The image below has the solution. To read the text, enlarge it.

Is O2 (oxygen gas) a molecule
Is H2O (water) a molecule
Is N2 (nitrogen gas) a molecule
Is CO2 (carbon dioxide gas) a molecule
Is CH4 (methane gas) a molecule
this is 5th grade science btw!

Answers

they’re all molecules because molecules are groups of atoms bonded together

which statement characterizes commensalism?

a) both organisms are negatively affected.
b) one organism benefits while the other organism experiences harm.
c) one organism benefits while the other organism is not affected.

Answers

Answer:

c) one organism benefits while the other organism is not affected.

One organism benefits while the other organism is not affected

Which of the following physical features would characterize an area that has low access to freshwater or limited rainfall?
O Dry
O Wet
O Cold
O Mountainous

Answers

Answer:  O Dry

Explanation:

Answer:

The answer would be dry.

Explanation:

It would be dry because, let’s say it was the desert… the dessert does not get much rainfall. Also it has barely any access to freshwater


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What happens when human DNA is inserted into a bacterial plasmid?

Answers

Answer:

creates a so-called recombinant plasmid

Researchers can insert DNA fragments or genes into a plasmid vector, creating a so-called recombinant plasmid. This plasmid can be introduced into a bacterium by way of a process called transformation. Then, because bacteria divide rapidly, they can be used as factories to copy DNA fragments in large quantities.

What is inserting a human gene into a bacterial plasmid?

Recombinant DNA is a technology scientist developed that made it possible to insert a human gene into the genetic material of a common bacterium.

What is it called when DNA is inserted into a plasmid?

In a typical cloning experiment, researchers first insert a piece of DNA, such as a gene, into a circular piece of DNA called a plasmid. This step uses restriction enzymes and DNA ligase and is called ligation.

Thus, by inserting human DNA into a bacterial plasmid, bacteria divide rapidly and they can be used as factories to copy DNA fragments.

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Which of the following compounds can be classified as a proteins?

Answers

Where’s the picture?

A parallel circuit ha a 12V power pack. It ha 2 branche each with a light bulb on. What i the potential difference of each of the light bulb?

Answers

The potential difference between each of the light bulbs will be 6.

According to ohms law,

V=IR

V=12v

R=2 (2 branches draw current so they serve as resistance)

I=V/R

=12/2

=6 A

This current will serve as the voltage difference between each light bulb.

The potential difference may be a degree of the sum of vitality exchanged between two points in a circuit. Potential contrast is additionally known as voltage and is measured in volts (V).

The potential difference over a component is measured by employing a voltmeter set in parallel with the component. Voltage, moreover, known as electric weight, electric pressure, or (electric) potential contrast, is the distinction in electric potential between two focuses.

In an inactive electric field, it compares to the work required per unit of charge to move a test charge between the two focuses.

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Why does a shaved camel standing in the sun require more water than an unshorn camel?.

Answers

In order to lower the bog, sweat essentially cools the air around the body as it evaporates. The shaved camel needs more water because it dries out more quickly than an onshore camel.

What justifies the process of evaporation?

The process of evaporation causes a liquid to become a gas. The phenomenon is easy to visualize when raindrops "vanish" on a hot day and when wet clothing dry in the sun. In these situations, the liquid water isn't truly dissipating; instead, it's evaporating into such a gas known as the water vapor. We experience global evaporation.

Why is it called evaporation?

Evaporation occurs on surfaces. Evaporation is a surface phenomenon because it occurs when molecules with higher kinetic energy from the top layer of the liquid escape into the air.

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What defines a substantial increase in a jellyfish population within a short time?.

Answers

As a result of a greater reproductive rate, a jellyfish bloom is described as a significant rise in a jellyfish bloom within a short period of time.

What drives the growth in the number of jellyfish?

Because jellyfish can survive with less oxygen than many other marine animals, the rise in their population has been linked to hotter waters and even increasing pollution.

What does it signify when there are many jellyfish present?

The recent period of sunny skies and southerly winds may have increased the amount of nutrients in the water, enhancing the conditions for jellyfish repopulation. One aspect that contributes to enormous jellyfish spawning like those is eutrophication, which is the scientific word for an overabundance of nutrients.

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The nervous tissue outside of the central nervous system composes the ________ nervous system.

Answers

The nervous tissue outside of the central nervous system composes the peripheral nervous system.

Nervous tissues are the tissues found in the brain, spinal cord and also the nerves. There are two main types of cells in the nervous tissue: nerve cells and glial cells. Their function is to regulate all the body functions by the receiving and transmission of signals.

Peripheral nervous system is a type of nervous system which is further subdivided into: autonomic nervous system and somatic nervous system. The components of peripheral nervous system are the nerves that branch off from the spinal cord and extends to the whole body.

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compared with cells that are surrounded by a lipid membrane, viruses . . . choose one:a. may be surrounded by a membrane synthesized by viral proteins.b. may be surrounded by a membrane derived from the host cell.c. are also always surrounded by a lipid membrane.d. are never surrounded by a lipid membrane.

Answers

Not even all viruses have an envelope-like membrane surrounding the capsid. Viruses do have a wall outside of the capsid, but it originates from the host.

What do viruses possess?

A virus's outer layer is formed of a nutrient coat that serves as the nucleus of its mitochondrial Genome, either RNA or Genetic material. The second spikey coat of the mailbox is occasionally seen around the virus. Viruses are able to attach to and enter new hosts.

How do viruses become made?

It's probable that mobile genetic elements that had the ability to move between cells gave rise to viruses. They might be related to organisms that were once free-living but later switched to a parasitic mode of reproduction. It's possible that viruses existed before cellular life developed and helped to shape it.

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what percentage of offspring will have a homozygotes dominate dominant genotype?
0 percent
100 percent
25 percent
50 percent

Answers

Answer:

100 percent

Explanation:

how does the central nervous system integrate incoming information

Answers

Answer:

Explanation:

The brain and the spinal cord make up the central nervous system. The nervous system is the most complex and highly organized body system. It receives information from the sensory organs via nerves, transmits the information through the spinal cord, and processes it in the brain.

Via Nerves

Through the spinal cord and nerves, it carries information from the sensory organs to the brain, where it is processed.

which of the following would be the first step in biosynthesis of a virus with a - (minus) strand of rna?
a. synthesis of protein from - strand RNA used as a template
b. synthesis of DNA from - strand RNA used as a template
c. synthesis of + stranded RNA from - strand RNA used as a template
d. synthesis of - stranded RNA from - strand RNA used as a template

Answers

Answer: Synthesis of double-strand RNA from an RNA template.

Explanation:

In an experiment, you test whether the number of times you rub a cloth against a balloon changes how long it will stick against the wall. You start by rubbing the balloon 10 times with the cloth and stick it to the wall. You repeat this three times and record your results in a table.


Observation
Number of Times Balloon is Rubbed by Cloth Trial #1 Trial #2 Trial #3
10 6 seconds 20 seconds 20 seconds


What statement best reflects why you should do repeated trials in an experiment?
Rubbing the balloon against a cloth 10 times produces very different results each time it is repeated.
The first trial is right because it is done with new materials that give the best results.
The second and third trials were wrong because they were very different from the first trial.
You would make a wrong conclusion if it were based on the first trial in the experiment.

Answers

The statement that best reflects why you should do repeated trials in an experiment is:

You would make a wrong conclusion if it were based on the first trial in the experiment; option D.

What are experiments?

Experiments are tests or procedures performed to test a given hypothesis or theory about a given observation.

In performing experiments, the procedure usually involves the repetition of steps in the reaction.

Repetition is necessary in order to remove bias in measurements and to lower sampling and random error.

The steps in conducting experiments include the following:

stating the research questionstating the aim of the experimenetmaterial and methodsresults ad discussionconclusion

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