The MW of rGFP in the band can be determined by comparing the mobility of the band with that of protein standards run on the same gel.
The Western blotting technique is utilized to identify and detect specific proteins in a sample of tissue or extract. If there is a band in the W2 lane of the Western blot, one can conclude that rGFP is present in that band. The physical protein structure of rGFP could not be inferred from the presence of a band in the W2 lane of the Western blot. This requires additional analysis such as X-ray crystallography, nuclear magnetic resonance, or cryo-electron microscopy to analyze protein structure. MW is the molecular weight which can be determined using a molecular weight marker that runs in a parallel lane to the protein extract on the gel. In the Western blotting method, SDS-PAGE is typically used to separate proteins based on their size. The SDS-PAGE gel is calibrated with protein markers that have a known molecular weight. Hence, the MW of rGFP in the band can be determined by comparing the mobility of the band with that of protein standards run on the same gel.
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What is the relationship between temperature and volume if pressure is held constant?
A. inverse
B. direct
Answer:
A. inverse
Explanation:
Boyle's Law is a relationship between pressure and volume. In this relationship, pressure and volume have an inverse relationship when temperature is held constant. If there is a decrease in the volume there is less space for molecules to move and therefore they collide more often, increasing the pressure.
(Please help 15 points the assignment is late) (multiple questions) (everything is virtual task )
Part 1 What do you think will happen when an effervescent tablet is placed in the water under each inverted glass?
Part 2 Note the changes in the water level and the air space in both glasses, and write down your observations.
Part 3 Did your prediction match your observation?
Part 4. When a substance easily dissolves in a liquid, it has high solubility in that liquid. When a substance does not easily dissolve in a liquid, it has low solubility in that liquid. Solubility of a gas in water describes how well the liquid can “hang on” to gas, instead of releasing it into the air. Based on the results of your experiment, do you think that CO2 has a higher solubility in hot water or cold water? Why?
Part 5 Soda pop is carbonated with CO2. Mark puts one bottle of soda pop in the refrigerator and leaves the other out in the hot sunlight. After one hour, he opens both bottles. Which bottle will likely have more fizzing and bubbles? Why?
Part 6 One result of climate change is that ocean temperatures are increasing. If the temperatures continue to rise, what effect will that have on the oceans’ ability to retain CO2? How might this change affect the atmosphere?
The dissolution of gases in water is exothermic therefore oceans will dissolve less CO2 as their temperatures rise.
What is effervescence?
The term effervescence refers to the rise of a gas in a system or simply put, it is the evolution of gas. When an effervescent tablet is placed in the water under each inverted glass, a gas will rise in each glass.
The air space in the hot glass is greater than the air space in the cold glass We can see that the gas has a lower solubility in hot water than in cold water. This is because, solubility of gases is exothermic.
The bottle that is left in the hot sun will have more fizzing because the CO2 does not dissolve owing to the higher temperature.
The rise in ocean temperature means that the oceans will dissolve less CO2 in the future and reduce the CO2 holding capacity of the oceans.
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how is liming a lake similar to a doctor prescribing medicine for a patient?
Atomic Size: Determine which of the following element pairs is the larger one
1. Carbon or Fluorine
2. Iodine or Fluorine
3. Krypton or Argon
4. Hydrogen or Potassium
Why is it dangerous to have electric appliances near you when you are taking a bath?
Answer:
Because water conducts electricity, so if an electrical current were to meet the water while you're in there taking a bath, you would essentially be surrounded by a huge conductor.
what is motion of a force in kinetic energy
Answer:
Kinetic energy, form of energy that an object or a particle has by reason of its motion. If work, which transfers energy, is done on an object by applying a net force, the object speeds up and thereby gains kinetic energy.
why is it necessary to make sure the stopcock is in the open position and then immediately replace the stopper and syringe assembly in the Erlenmeyer flask after adding the hydrochloric acid to the marble chips?
It is important to keep the stopcock open after adding hydrochloric acid to the marble chips in an Erlenmeyer flask for gas collection and accurate measurement of the carbon dioxide produced during the reaction.
Why is it necessary to make sure the stopcock is in the open position and then immediately replace the stopperBy allowing the gas to escape into the syringe or collection apparatus, the experiment can obtain precise gas volume measurements. Additionally, immediately replacing the stopper and syringe assembly helps prevent gas loss and maintains safety by avoiding the build-up of excessive pressure inside the flask.
These measures ensure the reliable and controlled execution of the experiment while obtaining accurate data.
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The coffee shop near the local college normally sells 10 ounces of roasted coffee beans for $10. But the shop sometimes puts the beans on sale. During some sales, it offers "33 percent more for free." Other weeks, it takes "33 percent off" the normal price. After reviewing the shop's sales data, the shop's manager finds that "33 percent more for free" sells a lot more coffee than "33 percent off." Are the store's customers making a systematic error? Which is actually the better deal? a. No, they are not making a systematic error because "33 percent more for free" is the better deal. b. Yes, they are making a systematic error because "33 percent off" is the better deal. c. This cannot be determined from the information given.
The “33 percent more for free” deal is better than the “33 percent off” deal. This is because the “33 percent more for free” deal gives you 13.3 ounces of coffee for $10, while the “33 percent off” deal gives you only 6.7 ounces of coffee for $6.67
For the “33 percent more for free” deal:
10 dollars for 10 ounces of coffee
13.3 ounces of coffee for 10 dollars (33% more)
Price per ounce = 10/13.3 = $0.75 per ounce
For the “33 percent off” deal:
10 dollars for 10 ounces of coffee
6.7 ounces of coffee for $6.67 (33% off)
Price per ounce = 6.67/6.7 = $0.99 per ounce
So, the “33 percent more for free” deal is better than the “33 percent off” deal because you get more coffee for your money.
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help me........................ please?
Answer:
2 NO2 -> 2 O2 + N2
is a decomposition reaction
.Rank the following compounds in order of increasing viscosity at a given temperature. CH3OH, CH3CH3, H2C=O
A) H2C=O < CH3CH3 < CH3OH
B) CH3CH3 < H2C=O < CH3OH
C) CH3OH < CH3CH3 < H2C=O
D) H2C=O < CH3OH < CH3CH3
The correct answer is D) H2C=O < CH3OH < CH3CH3Explanation:Viscosity is the measure of the resistance of a liquid to flow.
It is the ratio of shear stress to shear rate, which is a measure of how fast the fluid is moving between the plates. It can be measured in different units such as poise or centipoise.The viscosity of a liquid is affected by the attractive forces between the molecules, which depend on the type of molecules and their intermolecular forces. The stronger the intermolecular forces, the more viscous the liquid.In this case, CH3OH (methanol), CH3CH3 (ethane), and H2C=O (acetone) are the compounds to be ranked. Methanol is a polar molecule with hydrogen bonding and has a higher viscosity than ethane, which is nonpolar and has weak van der Waals forces. Acetone is a polar molecule with dipole-dipole interactions that are intermediate between methanol and ethane. The order of increasing viscosity is therefore H2C=O < CH3OH < CH3CH3.
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Which is a characteristic of a physical change?
a
The chemical bonds in the substance are unchanged.
b
The change cannot be seen.
c
The molecules all move at the same speed.
d
The substance has reacted with another substance.
PLEASE HELP ME NO LINKS
Answer: I don’t think the answer is A or C. Maybe B.
The word chemosynthesis means
the synthesis of organic compounds by bacteria or other living organisms using energy derived from reactions involving inorganic chemicals, typically in the absence of sunlight.
Explanation:
What is the final concentration of a solution prepared by using 75.0 mL of 18.0 M H2SO4 to prepare 500. mL of solution?
A) 2.35 M
B) 8.33×10-3 M
C) 15.7 M
D) 2.70 M
E) 1.20 M
The required correct answer is D) 2.70 M
The final concentration of a solution prepared by using 75.0 mL of 18.0 M H2SO4 to prepare 500. mL of solution is 2.70 M. Here's how to calculate it:We will use the formula:M1V1 = M2V2where:M1 = initial molarityV1 = initial volumeM2 = final molarityV2 = final volumeGiven that M1 = 18.0 M, V1 = 75.0 mL, and V2 = 500. mL. We can find M2 as follows:M1V1 = M2V218.0 M x 75.0 mL = M2 x 500. mLM2 = (18.0 M x 75.0 mL)/500. mL= 2.70 MTherefore, the final concentration of the solution is 2.70 M. The correct option is D.
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A gaseous mixture contains 5.0 moles of nitrogen and 10.0 moles of helium . What is the total pressure if the temperature is 25 degrees C and the volume is 3.0 L? a) 122 atm
B) 56 atm
d) 2.0 atm
C)3.0 atm
Answer:
A) 122 atm
Explanation:
PV = nRT
Solve for P --> P = nRT/V
n = 10.0 mol + 5.0 mol = 15.0 mol
R = 0.08206 L atm / mol K
T = 25 + 273 = 298 K
V = 3.0
P = (15.0)(0.08206)(298) / (3.0) = 122 atm
Please Help
How many grams of carbon dioxide (CO2) are produced from 85.0 grams of carbon (C)?
Answer: If one mole of carbon monoxide has a mass of 28.01 g and one mole of carbon dioxide has a mass of 44.01 g , it follows that the reaction produces 44.01 g of carbon dioxide for every 28.01 g of carbon monoxide.
Explanation: (May Be Unrelated?)
An organism that makes or creates their own food?
A. Consumer
B. Producer
C. Decomposer
I think producer
option is A
Answer:
B. Producer
Explanation:
A producer is normally a type of plant which is food for the animals. Decomposers are things that decompose dead living things into nutrients and etc. Consumers are the ones who normally feed on producers in the food web and are small animals.
Predict whether the entropy change for this process is positive or negative and explain your answer. Then, predict whether the free energy change for the process is positive or negative and explain your answer.
The given question is incomplete. The complete question is:
Consider the spontaneous dissolution of NaCl in water: NaCl(s) → Na+(aq) + Cl −(aq) Predict whether the entropy change for this process is positive or negative and explain your answer. Then, predict whether the free energy change for the process is positive or negative and explain your answer.
Answer: The entropy change is positive. The free energy change for the process is negative and reaction is spontaneous.
Explanation:
Entropy is defined as the degree of randomness of a system. For a system in which randomness increses , the enetropy is said to increase and [tex]\Delta S[/tex] is positive.
As in the given reaction : [tex]NaCl(s)\rightarrow Na^+(aq)+Cl^-(aq)[/tex] , the solid is dissociating to give ions , randomness increases and thus entropy is positive.
Also the dissociation of a molecule requires energy , thus the enthalpy [tex]\Delta H[/tex] is also positive as heat is absorbed by the system.
[tex]\Delta G=\Delta H-T\Delta S[/tex]
[tex]\Delta G=(+ve)-T(+ve)[/tex]
[tex]\Delta G=-ve[/tex] when Temperature is high.
Consider the spontaneous dissolution of NaCl in water: NaCl(s) → Na+(aq) + Cl −(aq)
Predict whether the entropy change for this process is positive or negative and explain your answer. Then, predict whether the free energy change for the process is positive or negative and explain your answer.
The given question is incomplete. The complete question is:
Consider the spontaneous dissolution of NaCl in water: NaCl(s) → Na(aq) Cl −(aq) Predict whether the entropy change for this process is positive or negative and explain your answer. Then, predict whether the free energy change for the process is positive or negative and explain your answer.
Answer: The entropy change is positive. The free energy change for the process is negative and reaction is spontaneous.
Explanation:
Entropy is defined as the degree of randomness of a system. For a system in which randomness increses , the enetropy is said to increase and is positive.
As in the given reaction : , the solid is dissociating to give ions , randomness increases and thus entropy is positive.
Also the dissociation of a molecule requires energy , thus the enthalpy is also positive as heat is absorbed by the system.
when Temperature is high.
Which of these ideas did you include in your answer?
✔️ When a crystalline solid dissolves, it becomes more disordered.✔️ When disorder increases, ΔG is positive.✔️ This process probably has a positive ΔS .✔️ The process is spontaneous.✔️ Spontaneous processes have ΔG < 0; the process has a negative ΔG .
Have a Nice Day .
Both the appearance and cooking times of food items are affected by the way a piece of food is:
Select one:
a. examined.
b. tenderized.
c. purchased.
d. cut.
Answer:
D.
cut.
Explanation:
Both the appearance and cooking times of food items are affected by the way a piece of food is cut. Thus option D is correct.
what are the types of food ?
Food has an important role in both culture and cuisine of every country, Different types of food combine to form culinary delights which can bring people together
Food is obtained from both plants and animals where Plants are the primary producers in the food pyramid and all organism directly or indirectly obtain food from plants.
Herbivorous animals get their food from their plant ecosystem where Some animals, obtain food by hunting other animals while others depend on decaying matter of plants and animals which are called decomposers like bacteria, fungi.
Food offers variety forms depend upon climatic conditions and availability and depend on the different factors like Physical determinants ,Biological determinants, Economical determinants.
Thus option D is correct.
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Identify the element for this electron configuration: 1s^2 2s^22p^63s^23p^63d^104s^24p.
The element for the given electron configuration 1s²2s²2p⁶3s²3p⁶3d¹⁰4s²4p⁰ is Chromium (Cr).
An element's electron configuration is a numeric representation of how the electrons are distributed among the various energy levels and orbitals. It may provide valuable insights into an element's chemical properties and reactions. To comprehend this electron configuration, we'll go through each sublevel from the lowest to the highest energy level: 1s², 2s², 2p⁶, 3s², 3p⁶, 3d¹⁰, 4s², and 4p⁰.
The 1s sublevel contains two electrons (1s²). In the second energy level, the 2s sublevel contains two electrons (2s²), and the 2p sublevel contains six electrons (2p⁶). The 2p sublevel has three orbitals, each of which may accommodate two electrons. As a result, the 2p sublevel contains six electrons. The third energy level contains 3s² and 3p⁶ sub levels. The 3s sublevel contains two electrons, while the 3p sublevel contains six electrons.
Both sublevels' orbitals may accommodate two electrons. The 3d sublevel is also in the third energy level. It contains ten electrons (3d¹⁰). The 3d sublevel has five orbitals, each of which may hold two electrons. As a result, the 3d sublevel has ten electrons. The 4s sublevel is in the fourth energy level. It contains two electrons (4s²). Finally, the 4p sublevel contains zero electrons. As a result, the electronic configuration 1s²2s²2p⁶3s²3p⁶3d¹⁰4s²4p⁰ is that of Chromium (Cr).
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WILL RATE BRAINLIST
Your friend works in a bookstore where lab books for science are assembled. Each lab book uses 2 covers, 50 sheets of lined paper, 25 sheets of graph paper and 3 staples. Her inventory includes 150 covers, 7500 sheets of lined paper and 3000 sheets of graph paper and 250 staples. What is the “limiting reactant?” How many complete lab books can be made? How much of each of the other reactants remain in excess?
Answer:
The limiting reactant is the covers.
We are left with 3750 sheets of lined paper, 1125 sheets of graph paper and 25 staples.
Explanation:
the reaction is:
B - book
C - cover
Sl = sheet of lined paper
Sg - sheet of graph paper
St - staple
1B = 2C + 50Sl + 25Sg + 3St
We have covers for 150/2 = 75 books
We have lined paper for 7500/50 = 150 books
We have graph paper for 3000/25 = 120books
We have Staples for 250/3 = 83.(3) books, so for 83 books as we cannot make 1/3 of a book.
Because we only have covers for 75 books, covers are the limiting reactant.
After making 75 books we are left with:
0 covers
7500 - 75*50 = 3750 sheets of lined paper
3000 - 75*25 = 1125 sheets of graph paper
250 - 75*3 = 25 staples
The limiting reactant should be the covers.
We are left with 3750 sheets of lined paper, 1125 sheets of graph paper, and 25 staples.
Calculation of the limiting reaction:
The reaction should be like:
B - book
C - cover
Sl = sheet of lined paper
Sg - sheet of graph paper
St - staple
Now the equation should be
1B = 2C + 50Sl + 25Sg + 3St
We have covers for 150/2 = 75 books
We have lined paper for 7500/50 = 150 books
We have graph paper for 3000/25 = 120books
We have Staples for 250/3 = 83.(3) books, so for 83 books as we cannot make 1/3 of a book.
Now the left should be
7500 - 75*50 = 3750 sheets of lined paper
3000 - 75*25 = 1125 sheets of graph paper
250 - 75*3 = 25 staples
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Which statement about elements is true?
All substances are made up of elements.
There are approximately 50 elements.
An element is made up of different types of atoms.
Most elements are nonmetal.

Answer:
first one is right.
there are 117 elements, so 2nd option is wrong.
elements are made up of one type of atoms, so 3rd is also wrong.
most elements are metal. so 3rd is wrong
If the temperature remains constant and pressure is INCREASED on gas, what will happen to the volume?
The volume will increase.
The volume will decrease.
The volume will not change.
The volume will randomly increase and decrease, and keep doing this.
Answer:
I believe the volume should increase.
How many molecules are contained in 125 grams of oxygen
gas (O2)?
Answer:
i think its 62.50 :) hope this helped you out
Use the drop-down menu to complete the statement. This is why they have similar Atoms of elements in the same group have the same number of chemical properties.
Answer:
valence electrons
Explanation:
i just took the test
How many grams are in 2.33 x 1023 molecules of Fe203?
61.9 grams
2.33x10^23 molecules (159.7 g Fe2O3/6.02x10^23 molecules) = 61.9 g
The structural layers of the sun are shown in the cross-sectional diagram. An image of six concentric circles is shown. The six layers thus formed are of varying thickness. The layers in order from inside out are labeled, A, B, C, D, E, F. Which layer represents the radiative zone? A B C D (21 points plus brainliest!)
A layer represents the radiative zone which is at the centre of the cross-sectional diagram.
What is a radio active zone?The layer of a star that lies just outside the core, to which radiant energy is transferred from the core in the form of photons.
In the Sun, the region between the solar core at 0.2 of the Sun's radius and the outer convection zone at 0.71 of the Sun's radius is referred to as the radiation zone, although the core is also a radiative region.
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B
A is inner core B is outer core and there is a few other layers but from what I know looking at this the second layer has always been the radioactive zone.
If you get this right I would love brainiest
Mg(s) + HCl (l) --> H2 (g) +MgCl2 (s) A student conducted an experiment with 2g of magnesium and 3 mL of 6M hydrochloric acid. Equal volumes (3mL) of hydrochloric acid and magnesium strips (2g) were put into each tube. What are the reactants in this reaction?
Answer: Do you have an image so I can solve it?
30g of Aluminum at 80°c is added to 30g of water at 10°c . Predict
the final temperature of the water
Answer:
the explanation of this 20
Explanation:
20 c
How many moles are in 19.82 g Mg?
Help me pleaseeeeeeeeeee
Answer:
I think A I hope it's A
Explanation:
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