How do you remove bacterial contamination from cell culture?

Answers

Answer 1

Bacterial contamination can be removed from cell culture using several methods. These include proper aseptic technique, filtration, antibiotics, and centrifugation.

The first and most important method for removing bacterial contamination is ensuring proper aseptic technique. This includes using sterile equipment and materials, wearing gloves and masks, and cleaning all surfaces and equipment with a disinfectant. Additionally, it is important to use aseptic techniques when transferring media and culturing cells. Following these basic techniques can help to reduce the risk of bacterial contamination from the start.

In the event of contamination, several other methods can be used to remove bacteria from culture. One of the most common methods is filtration. This involves passing the culture through a filter to remove the bacteria. This method is effective but may be time-consuming, depending on the size of the culture and the particle size of the bacteria.

Another method for removing bacteria from cell culture is antibiotics. Antibiotics can be added directly to the culture to kill the bacteria, though this may not be ideal in some cases due to the potential to affect the cells. Additionally, antibiotics may not be effective against certain types of bacteria. Therefore, it is important to select an appropriate antibiotic based on the type of bacteria present.

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Related Questions

What would happen within a few months if all de composers on earth disappeared overnight I’ll leave a picture here

Answers

there would be an over abundance of organic waste.

A cell is placed into a solution and is left sitting for 24 hours. When you come back you notice the cell is exactly the same size it was beforehand. Which type of solution was the cell sitting in? Explain

Answers

Answer:

Isotonic Solution

Explanation:

There are commonly three types of solutions:

Hypertonic solution = the concentration of solute is greater than in the cellHypotonic solution = the concentration of solute is less than in the cellIsotonic solution= the concentration of solution and cell is same.

So, if the cell was in any other solution, it would have been burst or it would shrink. but nothing changed because it was in a solution where outside environment is similar to the internal environment

(04.05 MC)
Which claim best supports the cycling of carbon through the spheres of Earth?

Answers

Answer:

Burning fossil fuels adds the necessary amount of carbon in the atmosphere for cellular respiration to take place

PLEASE HELP NO LINKS PLEASE

Which term best completes the diagram below?

nucleus
cell
molecule
O muscle

Answers

It’s Cells because cells make up tissues

Some animals hunt by _________, or sneaking up on, their prey. M is the second letter.

Answers

Answer:

Ambush

Explanation:

Ambush is a sneaky attack/Surprise attack

Ambush most likelyyy

My bird recently flew into a window and in bleeding between his nostrils. The wound keeps bleeding, and we can't afford a Vet visit right now, what should I do?

Answers

Answer:

he finna die

Explanation:

Answer:

OMG are the wings okay

wrap the bird up in an bandage cloth if you have any!

Explanation:

Cells: Question 10
Which of these structures would be classified as an organ?
Select one:
A)A ciliated cell within the respiratory tract
B)Multiple types of tissues that work together to filter wastes from the blood
C)A liquid that contains proteins, salts, and water
D)A group of square-shaped cells surrounding a tubule

Answers

Answer:

The answer would be B.

Explanation:

An organ is a group of cells that form tissue which then make up an otgan. A group of Tissues is an Organ and tgat orgab serves a specific function in the body. The group of tissues is the organ, and the filter of waste from the blood is the function.

how is modern earth different from earth over four billion years ago?

Answers

4 billion years ago the temperature was much higher due to repeated volcanoes and hot gases present in the atmosphere. There weren't any live forms on earth. ... The atmosphere had a different concentration of gases on the earth. There were no life sustaining gases.

Mendel found out everything about genetics and we have not learned anything new about genetics in the last 160+ years. true or false?

Answers

Answer:

false

Explanation:

there has been many advancements in genetics ever since Mendel's discoveries

In pea plants, peas can be green or yellow in color. Assume that green peas are dominant to yellow peas. Is it possible for two plants with green peas to produce any offspring with yellow peas?
vocabulary words to use in response: dominant allele, recessive allele, , heterozygous, genotype, phenotype

Answers

Answer:

No.

Explanation:

Turns out they have yellow peas because the yellow version, Y, is dominant over the recessive green version, y. Y always wins out over y: When a pea plant makes seeds, only one of its two copies of the color gene goes into the seed. So if you have two green pea plants, each can only pass a green version.

Answer:

No.

Explanation:

Turns out they have yellow peas because the yellow version, Y, is dominant over the recessive green version, y. Y always wins out over y: When a pea plant makes seeds, only one of its two copies of the color gene goes into the seed. So if you have two green pea plants, each can only pass a green version.

The diversity of dogs today can be attributed to selective breeding,
O True
O False

Answers

The answer would be true

What is the main body system affected from sickle cell anemia?

Answer choices:

Digestive System

Nervous system

Circulatory System

Respiratory system

Muscular System

Skeletal System

Answers

Answer:

Circulatory system

Explanation:

It flows through your blood and it can affect your veins,arteries,heart,ect

CO
Ecosystem productivity is principally limited by
a. sunlight and nutrient availability
b. water and nutrient availability
the availability of time and nutrients
d. none of the above
C.
Please select the best answer from the choices provided
A
B

Answers

Answer:

a. sunlight and nutrients availability.

hope this helps you ☺️☺️

Answer:

A

Explanation:

Why are silent mutations possible for some of the amino acids?

Answers

Answer:

That's possible because proteins are encoded by "triplets" of nucleotides, each responsible for adding a particular amino acid to the protein chain. A change in one nucleotide, however, doesn't always change the triplet's meaning; the mutated triplet may still add the same amino acid.

Explanation:

- Eijiro <3

what are the energy-storing compounds in photosynthesis?

Answers

Answer:

Hi, Plants store their energy in the form of starch, which is a complex carbohydrate that can be broken down into a simple carbohydrate (glucose) for the plant to use for energy. Plant cells store starch in storage organelles as all cells do. (vacuoles).

Answer:

Photosynthesis is the process by which organisms that contain the pigment chlorophyll convert light energy into chemical energy which can be stored in the molecular bonds of organic molecules (e.g., sugars). Photosynthesis powers almost all trophic chains and food webs on the Earth.

is it wrong for parents to give female hormone pills to there little sons, without there consent?

Answers

Without consent, almost everything is wrong... so yes, it’s wrong.
Yes definitely nxnbbbb

Please help will give brainliest

Answers

Answer:

a. domain

Explanation:

I am assuming that you are looking for the largest system, in that case it is domain.

From this list, it goes

Domain>kingdom>phylum>taxonomy

hope this helps :3

4. The suns energy causes water to change from liquid to gas during the process of ..
A. precipitation
B. condensation
O C. evaporation
O D. Water cycle

Answers

the answer is evaporation

When is extinction of a species most likely to occur?
A. when environmental conditions change and the adaptive traits of the species favor the survival and reproduction of some of it's members
B. when environmental conditions remain the same and the proportion of individuals within the species that lack adaptive traits increases
C. when environmental conditions change and the members of the species lack adaptive traits to survive and reproduce
D. when environmental conditions remain the same and the proportion of individuals within the species that possess adaptive traits increases​

Answers

C. Species change over time

What do you think accounts for the higher risk of earthquakes in Los Angeles than in Chicago?

Answers

Los Angeles has the higher risk of earthquakes
Los angles is closer to the San Andreas fault. The San Andreas fault is a tectonic boundary between the Pacific plate in the North American plate.

why do you think farmers are not using cover cross?

Answers

Because the competition is too stressful for the weeds to handle , making it easier for farmers to have compete weed control

Please help ASAP EXTRA POINTS AND WILL-MARK BRAINLIEST!!!

How could the Galapagos finches have evolved into 13 different species ? (2-3 sentences)

Please help thanks

Answers

Answer:

they adapted to their environment, some have sharper beaks which are used to crack nuts, when they were introduced to the new environment they evolve to the new surroundings in order to survive. therefore, seeing as though there is 13 different species, the Galapagos islands have many different food sources, and ecosystems.  

Explanation:

Due to sun damage, a skin cell acquired a mutation in DNA sequence in a gene essential for cell survival. This mutation, however, was not passed on to the offspring of the organism. Which explanation best accounts for this observation?

Answers

Answer:

Only mutations in reproductive cells can be passed from parents to offspring.

Explanation:

some consumers such as many flying insects need large amounts of energy to grow and move from one place to another. how do consumers get energy to function?​

Answers

consumers get energy to function by consuming food from other organisms (animals), while producers get their own energy from themselves (plants)

If two-hybrid Ff rabbits mate, what will the offspring look like?

Answers

Answer:

25% FF - homogenous dominant

25% ff - homogeneous recessive

50% Ff - heterogenous

Explanation:

When two-hybrid Ff rabbits mate, then the offspring look like

25% FF - homogenous dominant25% ff - homogeneous recessive50% Ff - heterogenous.

What is Mating?

For the goal of sexual reproduction, opposite-sex or hermaphrodite organisms team together during mating. Two gametes join together during fertilisation. When two sexually reproducing creatures join their sex organs, internal fertilizations and insemination take place.

Punnett Square situations with two hybrid parents marrying are frequent. The heterozygous hybrid parents possess a single allele (A and a, Dominant and Recessive). There are four genotype outcomes that can happen if two hybrid parents mate.

Thus, when two-hybrid Ff rabbits mate, then the offspring look like

25% FF - homogenous dominant25% ff - homogeneous recessive50% Ff - heterogenous.

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How do animals help recycle carbon atoms?

Answers

Answer:

animals, oxygen combines with food in the cells to produce energy for daily activity and then gives off carbon. The carbon combines with oxygen to form carbon dioxide (CO2) and is released back into the atmosphere as a waste product when animals breathe and exhale

Answer:

In animals, oxygen combines with food in the cells to produce energy for daily activity and then gives off carbon. The carbon combines with oxygen to form carbon dioxide (CO2) and is released back into the atmosphere as a waste product when animals breathe and exhale.

Explanation:

The plant helps with which part of the nitrogen circle?

Answers

The stems of the cycle

Please Please Help Me!!!

Describe an experiment used to prove that amino acids and sucrose go up and down the phloem.​

Answers

Answer:

By using carbon-14 in the experiment in order to prove the statement.

Explanation:

Translocation occurs in the plant body in order to transport amino acids and sucrose from one part to another by the phloem tissues. In order to prove this, we have to incorporate radioactive element in the amino acid or sucrose which shows us that through which layer the amino acids and sucrose move in the plant body. For this purpose, carbon-14 can be used to trace the path of the translocation of amino acids and sucrose. Through this experiment we will find out that phloem layer is the path used by the plant to move amino acids and sucrose from one part to another.

4 1 Your friend, Luis, recently told you that bacteria started MAGICALLY growing on the inside of his shoes causing his feet to smell. You know this is not true based on which part of the cell theory? O cells are the basic units of life all living things are made of cells very few cells reproduce all cells come from pre-existing cells 5 To enter or leave a cell, molecules, like water, must pass through the​

Answers

Answer:

You have to finish putting the question

Explanation:

When yellow guinea pigs (Y) are crossed with white guinea pigs (W), cream-colored offspring (YW) are produced. Can a pet-store owner mate two cream-colored guinea pigs and get a white guinea pig?

Answers

Answer:

It is possible.

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